Immunohistochemical expression of Nanog protein in prostate cancer cells of distinct grade groups

Kudryavtsev GYu1,3, Kudryavtseva LV1, Mikhaleva LM2, Kudryavtsevа YaYu1, Solovyeva NA1, Osipov VA3, Babichenko II1
About authors

1 Peoples’ Friendship University of Russia, Moscow, Russia

2 Research Institute of Human Morphology, Moscow, Russia

3 Hospital for War Veterans № 2, Moscow, Russia

Correspondence should be addressed: Georgy Yu. Kudryavtsev
Baikalskaya, 35, apt. 216, Moscow, 107207; ur.liam@ahsogk

About paper

Compliance with ethical standards: the study was approved by the Ethics Committee of Medical Institute of the Peoples' Friendship University of Russia (protocol № 13 dated December 19, 2019); the study design was consistent with the Declaration of Helsinki guidelines regarding the research involving identifiable human tissue samples. The informed consent was submitted by all patients.

Author contribution: Kudryavtsev GYu, Kudryavtsevа YaYu, Solovyeva NA — literature survey, manuscript writing; Kudryavtseva LV, Osipov VA — statistical data processing; Mikhaleva LM — sample collection and processing; Babichenko II — study concept and design.

Received: 2020-10-06 Accepted: 2020-10-20 Published online: 2020-10-31

Prostate cancer is the most common type of cancer among men, which is mainly due to extensive use of screening tests and high total number of prostate biopsies. Verification of tumors with poorer prognosis is the primary goal of prostate cancer management. The study was aimed to determine the clinical and morphological associations and the prognostic value of the Nanog protein expression in prostate cancer of distinct Grade Groups. We used the prostate tissue specimens obtained during surgery, and the biopsy specimens, the total of 89 cases. Histological and immunohistochemical assessment was performed using antibodies to Ki-67 and Nanog. Correlations between the expression of markers and the Grade Groups were revealed using the Spearman's rank correlation coefficient, and the correlation with clinical and morphological characteristics was determined using the chi-squared test (χ2). There was a positive correlation between the expression of Ki-67 and Nanog, and the Grade Group numerical order (rs = 0.619, p < 0.001 and rs = 0.786, p < 0.001 respectively). We managed to find the relationship between the high Nanog expression and the extraprostatic extension (p = 0.041). High expression of Nanog protein in the prostate cancer cells was associated with a higher-grade adenocarcinoma and indicated a poor prognosis.

Keywords: prostate cancer, prostate adenocarcinoma, Ki-67, Nanog, Gleason index, Grade Groups