Local antioxidant effect of original dermal film with melatonin in thermal injury

Osikov MV1, Simonyan EV1, Ageeva AA1, Ageev YuI1, Fedosov AA2, Sinitsky AI1
About authors

1 South-Ural State Medical University, Chelyabinsk, Russia

2 Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Anna A. Ageeva
Vorovskogo, 64, Chelyabinsk, 454092; ur.xednay@r.aveega.enna

About paper

Compliance with ethical standards: the study was approved by the Ethics Committee of the South Ural State Medical University, Chelyabinsk (Minutes #10 of November 15, 2019), carried out in standard vivarium conditions with strict adherence to the requirements for animal keeping and care, as well as withdrawal of animals from the experiment and subsequent disposal in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental or Other Scientific Purposes (ETS № 123 of March 18, 1986, Strasbourg), EC Recommendations 2007/52/EC of June 18, 2007 outlining procedures of keeping and care for animals used for experimental and other scientific purposes, as well as the European Parliament and EU Council Directive 2010/63/EU of September 22, 2010 on protection of animals used for scientific purposes as governed by the rules of humane treatment of animals, guidelines for their withdrawal from experiments and euthanasia.

Author contribution: MV Osikov — study concept and design, integrated analysis of the data obtained, authoring, manuscript editing; EV Simonyan — experimental material collection, analysis of the data obtained; AA Ageeva — experimental material collection, statistical processing and analysis of the data obtained, authoring; YuI Ageev — experimental material collection, statistical processing and analysis of the data obtained; AA Fedosov — analysis of the results, manuscript editing; AI Sinitsky — experimental material collection, manuscript editing.

Received: 2020-10-03 Accepted: 2020-10-23 Published online: 2020-11-11

Oxidative stress (OS) escalation associated with thermal trauma (TT) and pleiotropic effects of melatonin (MT) suggest a study of protective properties of the latter when applied as part of a novel dermal film (DF) to skin burns. This work aimed to assess the content of OS markers in the skin subjected to experimental TT and treated with DF with MT. Third A degree TT (area of 3.5%) were modeled by immersing a patch of skin in boiling water. Twelve cm2 of DF with 5 mg/g of MT were applied daily for 5 days. The parameters calculated were wound's area and epithelializatiohon rate. The products monitored in the burn wound were lipid peroxidation (LPO) products in heptane and isopropanol phases of the lipid extract and protein oxidative modification (POM) products, the modification being spontaneous and metal-dependent. With TT in the wound, the content of secondary and end LPO products in heptane and isopropanol phases increased on the 5th and 10th days; the total content of POM products grew on the 5th day (primary products, neutral) and on the 10th day (primary and secondary products, neutral). Application of DF to a TT wound reduced the burn area, increased the epithelialization rate (by the 10th day, the median went from 1.90% to 6.57%; p < 0.05), reduced the content of secondary and end LPO products in isopropanol phase (by the 10th day, the median went from 0.007 to 0.004 u.o.i; p < 0.05), reduced the total content of OMP products, namely that of primary neutral products — on the 5th day, of primary and secondary neutral products — on the 10th day. With TT present in the context of MT application, the burn area showed presence of secondary LPO products in heptane and isopropanol phases, LPO end products in isopropanol phase, POM products in the wound (basic and neutral primary/secondary POM products).

Keywords: oxidative stress, thermal trauma, melatonin, dermal film