2020 / 06

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DOI: 10.24075/brsmu.2020.074

ORIGINAL RESEARCH

Interrelation between miRNA and mRNA expression in HT-29 line cells under hypoxia

Nersisyan SA1, Galatenko AV2,3, Maltseva DV1,4, Ushkaryov YuA1, Tonevitsky AG1,4
About authors

1 National Research University Higher School of Economics, Moscow, Russia

2 Lomonosov Moscow State University, Moscow, Russia

3 Moscow Center for Fundamental and Applied Mathematics, Moscow, Russia

4 Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia

Correspondence should be addressed: Diana V. Maltseva
Vavilova, 7, Moscow, 117321; moc.liamg@avestlamd

About paper

Funding: the work was supported financially by the Russian Science Foundation (agreement #17-14-01338).

Compliance with ethical standards: the study was approved by the Ethics Committee of Amur State Medical Academy (Protocol № 10 dated November 20, 2019); the study conformed with the guidelines for the medical research involving human subjects. Voluntary informed consent was obtained from all the participants.

Author contribution: SA Nersisyan and AV Galatenko — sequencing data processing, bioinformatic analysis, functional gene analysis, building the regulatory network of interactions, manuscript preparation; DV Maltseva — cell culturing work, preparation of samples for sequencing, manuscript preparation for publication; YA Ushkaryov — discussion of the results, article text review; AG Tonevitsky — research organization, analysis of the results, article manuscript preparation.

Received: 2020-10-26 Accepted: 2020-11-22 Published online: 2020-12-07
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Fig. 1. Differential expression of genes associated with hypoxia. A. Glycolysis/gluconeogenesis. B. HIF-1 signaling pathway
Fig. 2. Regulatory network of interactions between transcription factors (TF) and miRNA, induced by hypoxia. Rectangles are TFs, ellipses — miRNAs. Expression growths and drops are shown in green and red, respectively. Arrows indicate activation of the expression, T-shaped lines — its suppression
Table 1. Differentially expressed miRNAs
Note: * — positive and negative values indicate the cellular miRNA level drop or growth in response to hypoxia.
Table 2. MiRNAs showing differential expression patterns specific to the particular miRNA arms
Note: * — positive and negative values indicate the cellular miRNA level drop or growth in response to hypoxia.