ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
A method for rapid generation of model intestinal barriers in vitro
About authors
1 National Research University Higher School of Economics, Moscow, Russia
2 SRC Bioclinicum, Moscow, Russia
Correspondence should be addressed: Sergey V. Nikulin
Vavilova, 7, Moscow, 117321; moc.liamg@b.c.nilukin
About paper
Funding: the study was supported by the Russian Science Foundation, project № 16-19-10597.
Author contribution: Nikulin SV — cell culture work, preparation of samples for transcriptome analysis, data analysis, manuscript preparation; Poloznikov AA — analysis of transcriptome data, manuscript preparation; Sakharov DA — supervision, manuscript preparation.
Compliance with ethical standards: the study complies with the 1964 Helsinki Declaration and its later amendments.
Received: 2020-11-09
Accepted: 2020-12-03
Published online: 2020-12-15
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Fig. 1. TEER values in 24 hours (A) and 48 hours (B) from the onset of experiment. Statistically significant differences of coated membranes compared to uncoated membranes are marked with * COL IV — type IV collagen; LAM 332 — laminin-332; PET — uncoated
Fig. 2. Caco-2 monolayer images in 24 h (scale segment bar is 200 μm) COL IV — type IV collagen; LAM 332 — laminin-332; PET — uncoated
Table. Type IV collagen expression levels in differentiated and undifferentiated Caco-2 cells (Affymetrix logarithmic scale)
