2021 / 04

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DOI: 10.24075/brsmu.2021.034

ORIGINAL RESEARCH

Fluorescence detection of amyloid deposits in human tissues using histochemical dyes

Guselnikova VV1,2, Sufieva DA1, Tsyba DL1, Korzhevskii DE1
About authors

1 Institute of Experimental Medicine, Saint Petersburg, Russia

2 Saint Petersburg State University, St Petersburg, Russia

Correspondence should be addressed: Valeria V. Guselnikova
Acad. Pavlov, 12, Saint Petersburg, 197376; ur.xednay@aiirelaV.avocinlesuG

About paper

Author contribution: Guselnikova VV — literature analysis, study planning, staining specimens, analysis and interpretation of the results, manuscript draft writing; Sufieva DA — quantitative data analysis; Tsyba DL — quantitative data analysis; Korzhevskii DE — conceptual development, study planning, manuscript editing.

Compliance with ethical standards: the study was conducted in accordance with the requirements of the World Medical Association Declaration of Helsinki (2013) and approved by the Ethics Committee of the Institute of Experimental Medicine (protocol № 3/18 dated November 22, 2018).

Received: 2021-07-12 Accepted: 2021-07-25 Published online: 2021-07-31
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Fig. 1. Fluorescence detection of amyloid deposits in human cerebral cortex (А, C, E) and myocardium (B, D). Thioflavin T (А, B), Congo red (C, D) and methyl violet (E) staining. Arrow 1 points at amyloid plaque with dense central core, arrow 2 points at fibrillar amyloid plaque (no dense core), short arrow points at lipofuscin deposits, and the arrow head points at cell nucleus. The scale bar shows 50 µm (А, B, D) and 20 µm (C, E)
Fig. 2. Metachromatic staining of transthyretin amyloid in human myocardium. А. Methyl violet stain. B. Basic fuchsin stain. The scale bar shows 50 µm
Fig. 3. Quantitative assessment of amyloid plaques stained using various methods. The differences with the control group (immunohistochemistry) are considered significant when p = 0.01 (*) and p < 0.0001 (****)
Table 1. List of dyes used
Table 2. Results of β-amyloid staining in human cerebral cortex
Table 3. Results of transthyretin amyloid staining in human myocardium