ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Combined effects of bacteriophage vB_SauM-515A1 and antibiotics on the Staphylococcus aureus clinical isolates
About authors
Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
Correspondence should be addressed: Maria A. Kornienko
Malaya Pirogovskaya, 1а, Moscow, 119435; moc.liamg@ayiramokneinrok
About paper
Funding: the study was funded by the Russian Science Foundation, project number 22-15-00443, https://rscf.ru/project/22-15-00443/.
Acknowledgements: the authors express their gratitude to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of the Russian Federal Medical Biological Agency, for bacterial gene sequencing required for multilocus sequence typing of the strains.
Author contribution: Abdraimova NK, Kornienko MA — study plan, data acquisition and processing, manuscript writing; Bespiatykh DA — data processing, Kuptsov NS — data acquisition; Gorodnichev RB — study plan, data processing; Shitikov EA — data processing, manuscript writing.
Compliance with ethical standards: the study was carried out in accordance with the sanitary and hygienic guidelines SP 1.3.2322-08 “Safety of Working With Microorganisms of III-IV Groups of Pathogenicity (Danger) and Causative Agents of Parasitic Diseases”; sanitary and hygienic guidelines SP 1.3.2518-09 "Additions and Amendments № 1 to the guidelines SP 1.3.2322-08 "Safety of Working With Microorganisms of III-IV Groups of Pathogenicity (Danger) and Causative Agents of Parasitic Diseases"; sanitary and hygienic guidelines "Sanitary and Epidemiologic Requirements for the Handling of Medical Waste" (SanPiN 2.1.7.2790-10); Federal Clinical Guidelines "Rational Use of Bacteriophages in Clinical and Epidemiological Practice".
Received: 2022-09-23
Accepted: 2022-10-18
Published online: 2022-10-26
|
Currently, the search for new therapy options for infectious diseases caused by multidrug-resistant Staphylococcus aureus is a priority. Combining antibiotics with virulent (lytic) bacteriophages may be considered a viable alternative to conventional antibiotic therapy. The study was aimed to assess the combined effects of the lytic bacteriophage vB_SauM-515A1 of Herelleviridae family and antibiotics of various classes on the Staphylococcus aureus clinical strains. Strains (n = 4) belong to the clinically significant sequence types ST1, ST8, ST121 and are characterized by multidrug resistance. Efficiency of the combination use of two antibacterial agents was assessed by comparison of optical densities of the test samples and controls after 24 hrs. of incubation. Mutually enhancing activities of bacteriophage used in combination with oxacillin, tetracycline and linezolid were revealed, in contrast to the separate use of each agent. Efficiency generally increased with the selected optimum multiplicity of infection values. No antagonism was revealed when combining the phage with antibiotics. Thus, virulent bacteriophage vB_SauM515A1 can be considered as a possible auxiliary therapeutic agent for antimicrobial-resistant strains of Staphylococcus aureus.
Keywords: Staphylococcus aureus, vancomycin, levofloxacin, bacteriophage therapy, Herelleviridae, combined effects, gentamicin, tetracycline, oxacillin, linezolid