2022 / 05

Next Paper
DOI: 10.24075/brsmu.2022.052

ORIGINAL RESEARCH

Combined effects of bacteriophage vB_SauM-515A1 and antibiotics on the Staphylococcus aureus clinical isolates

Abdraimova NK, Kornienko MA, Bespiatykh DA, Kuptsov NS, Gorodnichev RB, Shitikov EA
About authors

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia

Correspondence should be addressed: Maria A. Kornienko
Malaya Pirogovskaya, 1а, Moscow, 119435; moc.liamg@ayiramokneinrok

About paper

Funding: the study was funded by the Russian Science Foundation, project number 22-15-00443, https://rscf.ru/project/22-15-00443/.

Acknowledgements: the authors express their gratitude to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of the Russian Federal Medical Biological Agency, for bacterial gene sequencing required for multilocus sequence typing of the strains.

Author contribution: Abdraimova NK, Kornienko MA — study plan, data acquisition and processing, manuscript writing; Bespiatykh DA — data processing, Kuptsov NS — data acquisition; Gorodnichev RB — study plan, data processing; Shitikov EA — data processing, manuscript writing.

Compliance with ethical standards: the study was carried out in accordance with the sanitary and hygienic guidelines SP 1.3.2322-08 “Safety of Working With Microorganisms of III-IV Groups of Pathogenicity (Danger) and Causative Agents of Parasitic Diseases”; sanitary and hygienic guidelines SP 1.3.2518-09 "Additions and Amendments № 1 to the guidelines SP 1.3.2322-08 "Safety of Working With Microorganisms of III-IV Groups of Pathogenicity (Danger) and Causative Agents of Parasitic Diseases"; sanitary and hygienic guidelines "Sanitary and Epidemiologic Requirements for the Handling of Medical Waste" (SanPiN 2.1.7.2790-10); Federal Clinical Guidelines "Rational Use of Bacteriophages in Clinical and Epidemiological Practice".

Received: 2022-09-23 Accepted: 2022-10-18 Published online: 2022-10-26
|
Fig. 1. Growth curves of the S. aureus infected with bacteriophage vB_SauM-515A1 with various MOI values
Fig. 2. Combined effects of the lytic bacteriophage vB_SauM-515A1 and antibiotics (oxacillin (А), tetracycline (B), linezolid (C)) on the S. aureus strains at optimum MOI values. Statistical significance: * — p ≤ 0.05; * * — p ≤ 0.01; * * * — p ≤ 0.001
Table 1. Characteristics of the Staphylococcus aureus strains
Note: R — resistant strains, I — strains showing intermediate resistance, S — susceptible strains.
Table 2. Resulting effects of the combination use of various vB_SauM-515A1 bacteriophage and antibiotic concentrations on the S. aureus clinical strains
Note: + — mutually enhancing activities; empty cell — lack of mutually enhancing activities; L — culture completely lysed by bacteriophage; S — antibiotic susceptible strain.