2022 / 06

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DOI: 10.24075/brsmu.2022.068

ORIGINAL RESEARCH

Effects of various mRNA-LNP vaccine doses on neuroinflammation in BALB/c mice

Kirshina AS1, Kazakova AA1, Kolosova ES1, Imasheva EA1, Vasileva OO1, Zaborova OV1,2, Terenin IM1,3, Muslimov AR1,4, Reshetnikov VV1,5
About authors

1 Research Center for Translational Medicine, Sirius University of Science and Technology, Sirius, Russia

2 Lomonosov Moscow State University, Moscow, Russia

3 Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia

4 St. Petersburg Pavlov State Medical University, St. Petersburg, Russia

5 Institute of Cytology and Genetics, Novosibirsk, Russia

Correspondence should be addressed: Vasily V. Reshetnikov
Olimpiyskiy prospekt, 1, Sochi, 354340, Russia; ur.hepsuitnalat@vv.vokintehser

About paper

Funding: the study was supported by the Ministry of Science and Higher Education of the Russian Federation (agreement № 075-10-2021-113, project ID RF-193021X0001).

Author contribution: Kirshina AS — RNA extraction, conducting PCR; Kazakova AA, Кolosova ES, Imasheva EA, Vasileva ОО — generating genetic constructs, RNA extraction, manuscript writing; Zaborova ОV — RNA formulation in LNP, manuscript writing; Terenin IM — RNA synthesis, manuscript writing; Muslimov AR — animal experiment, manuscript editing; Reshetnikov VV — animal experiment, data analysis, preparing illustrations, manuscript wr.

Compliance with ethical standards: the study was appoved by the Ethics Committee of Pavlov First St.Petersburg State Medical University (protocol № 83 of 21 September 2022); it was conducted in accordance with the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes (ETS No. 123, Strasbourg, 1986, with the 2006 Appendix), international convention on the humane treatment of animals (1986), Guide for the Care and Use of Laboratory Animals, 8th ed. (2010); Directive 2010/63/EU of the European Parliament and of the Council on the protection of animals used for scientific purposes (2010), Principles of Good Laboratory Practice (2016).

Received: 2022-12-01 Accepted: 2022-12-15 Published online: 2022-12-30
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Fig. 2. Expression of neuroinflammatory marker genes in the hypothalamus А. Astroglial and microglial response to acute inflammation. B. Relative expression of genes encoding pro-inflammatory cytokines (Il1β, Tnfa) and markers of glial activation (Aif1, Gfap) at various time points after the mRNA-LNP vaccine administration. The data are presented as mean ± standard error. * — p < 0.05, ** — p < 0.01, compared to the group that received normal saline at the same time point; # — p < 0.05, compared to the group that received 20 μg of mRNA-LNP at the same time point; $ — p < 0.05, $$ — p < 0.01, compared to the point within 8 hours after administration; & — p < 0.05, compared to the point within 4 hours after administration. Post hoc analysis using the Fisher’s LSD test
Table. Nucleotide sequences of primers and probes