2022 / 06

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DOI: 10.24075/brsmu.2022.066

ORIGINAL RESEARCH

Transgenic mice for study of the CDK8/19 cyclin-dependent kinase kinase-independent mechanisms of action

Stavskaya NI1, Ilchuk LA2, Okulova YuD2, Kubekina MV2, Varlamova EA2, Silaeva YY1, Bruter AV2
About authors

1 Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

2 Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

Correspondence should be addressed: Leonid A. Ilchuk
Prospekt Mira, 124, Moscow, 129164, Russia; moc.liamg@21kuhcel

About paper

Funding: RSF grant, project № 22-15-00227.

Author contribution: Stavskaya NI ― experimental procedure, working with animals; Ilchuk LA ― manuscript writing, design of genotyping systems, data analysis; Okulova YuD ― working with embryos; Kubekina MV ― preparation of genetically engineered construct, experimental procedure; Varlamova EA ― experimental procedure; Silaeva YuYu ― literature analysis, study planning; Bruter AV ― literature analysis, study planning, data analysis and interpretation, manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Commitee of the Institute of Gene Biology RAS (protocol No. 1 of 10 November 2021) and conducted in full compliance with the Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes.

Received: 2022-11-22 Accepted: 2022-12-19 Published online: 2022-12-28
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Fig. 1. F1 mouse genotyping by gel electrophoresis. The lanes with the bands of PCR products with the size of about 300 bp (fragment of STOP cassette) and about 400 bp (fragment of terminator) correspond to transgenic mice. The lanes with no bands correspond to the wild type mice. The first and the last lanes contain the size markers
Fig. 2. Scheme of the substrains obtained and copy numbers of the substrain members. The names of substrains are put in quotes. The average copy numbers of ansectors (F0) are put in parentheses. Only the copy numbers are provided for F1
Table 1. Olygonucleotide sequences
Table 2. Results of activities on generating primary transgenic animals