Development and characterization of a vector system based on the simian adenovirus type 25

Ozharovskaia TA, Popova O, Zubkova OV, Vavilova IV, Pochtovyy AA, Shcheblyakov DV, Gushchin VA, Logunov DYu, Gintsburg AL
About authors

Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation, Moscow, Russia

Correspondence should be addressed: Tatiana A. Ozharovskaia
Gamalei, 18, Moscow, 123098, Russia; ur.xednay@hzo.t

About paper

Funding: the work is the result of the effort under the "Development of a recombinant vaccines platform (including live vaccines) enabling design of the infectious diseases prevention vaccines" task set by the Ministry of Health of the Russian Federation.

Acknowledgments: we express our sincere gratitude to AE Nikonova, a researcher with the Laboratory of Molecular Biotechnology of the NF Gamaleya National Research Center for Epidemiology and Microbiology for her help in conducting the phylogenetic analysis of adenovirus hexon sequences, as well as to research laboratory assistant D.D. Kustova for her help in obtaining the whole genome sequences.

Author contribution: Ozharovskaia TA, Zubkova OV, Logunov DYu, Gintsburg AL — conceptualization and planning of the experimental part; Popova O, Zubkova OV — production of genetically engineered constructs and recombinant simian adenovirus type 25; Ozharovskaia TA, Vavilova IV — phylogenetic analysis, virological experiments; Pochtovyi AA — whole genome sequencing of adenoviruses; Ozharovskaia TA, Zubkova OV — article preparation and authoring, interpretation of the results; Shcheblyakov DV, Gushchin VA — manuscript editing; Gushchin VA — state assignment project management.

Compliance with ethical standards: the work was carried out in accordance with the principles of the Declaration of Helsinki.

Received: 2022-12-29 Accepted: 2023-01-20 Published online: 2023-02-28

Technological versatility and the humoral and cellular immune response induction capacity have conditioned wide spread of adenoviral vectors as vaccine and gene therapy drugs. However, vaccination with Sputnik V made a significant portion of the population immune to the types 5 and 26 (Ad5 and Ad26) recombinant human adenovirus vectors, which are some of the most frequently used bases for candidate vaccines. Today, vaccine designers tend to select alternative adenovirus serotypes as platforms to develop vaccines against new pathogens on. A good example is simian adenovirus type 25 (SAd25), which belongs to subgroup E. It is genetically distant from Ad5 and exhibits extremely low seroprevalence in human beings, which makes it an appealing alternative vaccine vector. The purpose of this work was to design and study a new vaccine platform based on simian adenovirus type 25. We relied on the advanced methods of molecular biology and virology to construct and make recombinant adenoviruses; the phylogenetic analysis in the context of this study was enabled with bioinformatic methods. The resulting recombinant adenoviral vector can effectively replicate itself in the HEK293 cell line (human embryonic kidney cells). This work substantiates the expediency of further investigation into the SAd25 vector as a platform for development of the prevention vaccines against various infectious diseases.

Keywords: adenovirus vector, vaccine platform, recombinant adenovirus, simian adenovirus type 25