Development and characterization of a vector system based on the simian adenovirus type 25

Ozharovskaia TA, Popova O, Zubkova OV, Vavilova IV, Pochtovyy AA, Shcheblyakov DV, Gushchin VA, Logunov DYu, Gintsburg AL
About authors

Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of the Russian Federation, Moscow, Russia

Correspondence should be addressed: Tatiana A. Ozharovskaia
Gamalei, 18, Moscow, 123098, Russia; ur.xednay@hzo.t

About paper

Funding: the work is the result of the effort under the "Development of a recombinant vaccines platform (including live vaccines) enabling design of the infectious diseases prevention vaccines" task set by the Ministry of Health of the Russian Federation.

Acknowledgments: we express our sincere gratitude to AE Nikonova, a researcher with the Laboratory of Molecular Biotechnology of the NF Gamaleya National Research Center for Epidemiology and Microbiology for her help in conducting the phylogenetic analysis of adenovirus hexon sequences, as well as to research laboratory assistant D.D. Kustova for her help in obtaining the whole genome sequences.

Author contribution: Ozharovskaia TA, Zubkova OV, Logunov DYu, Gintsburg AL — conceptualization and planning of the experimental part; Popova O, Zubkova OV — production of genetically engineered constructs and recombinant simian adenovirus type 25; Ozharovskaia TA, Vavilova IV — phylogenetic analysis, virological experiments; Pochtovyi AA — whole genome sequencing of adenoviruses; Ozharovskaia TA, Zubkova OV — article preparation and authoring, interpretation of the results; Shcheblyakov DV, Gushchin VA — manuscript editing; Gushchin VA — state assignment project management.

Compliance with ethical standards: the work was carried out in accordance with the principles of the Declaration of Helsinki.

Received: 2022-12-29 Accepted: 2023-01-20 Published online: 2023-02-28
Fig. 1. Phylogenetic tree showing the relatedness of the hexon (A), penton (B) and fiber (C) sequences of adenoviruses of different serotypes. The tree was built based on the amino acid sequences of hexons of 45 human adenovirus serotypes and 5 simian adenovirus serotypes. The evolutionary distances were calculated based on the number of differences; they are given in as amino acid differences per sequence. Ad5, Ad26 and SAd25 are highlighted in red
Fig. 2. Patterns of recombinant adenoviral vectors. A. Production of a plasmid construct containing the full-length genome of simian adenovirus type 25 and carrying the EGFP gene expression cassette. B. Genomes of recombinant replication-defective adenoviruses used in this study
Fig. 3. Cytopathic effect of adenoviruses on HEK293 cells 48 and 72 hours after transduction
Table. Biological and physical characteristics of recombinant adenoviruses