Macular rupture is among major vitreoretinal disorders resulting in significant visual function decline. In recent years, the use of platelet-rich autoplasma in the macular rupture surgery is considered to be promising. However, despite high rate of macular rupture closure (78–96%), the development of new technology for foveal microarchitectonics restoration and visual function improvement currently remains a pressing issue. The study was aimed to develop a new foveal reconstruction technique for macular rupture involving the use of autologous conditioned plasma. The reported study involved 9 patients (10 eyes) with idiopathic macular hole, who underwent standard three-port 27G vitrectomy involving the use of autologous conditioned plasma in accordance with the technique developed. The proposed technique makes it possible to achieve good clinical and functional surgical treatment outcome and ensures optimal and more manageable reconstruction of foveal microarchitectonics.