ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Preparation of a recombinant ribonuclease inhibitor in E. coli for use in mRNA synthesis in vitro
About authors
1 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia
2 Sirius University of Science and Technology, Sirius, Sochi, Russia
Correspondence should be addressed: Maxim O. Nagornykh
Prospekt Nauki, 5, Pushchino, 142290, Russia; moc.liamg@rennabred
About paper
Funding: the study was financially supported in the context of the program of Ministry of higher education and science of the Russian Federation (agreement #075-10-2021-113, unique project number RF----193021X0001).
Author contribution: Zakharov MV — selection of conditions of production of recombinant proteins, production experiments in different E. coli strains; Zagoskin AA — chromatographic clearing of recombinant proteins; Nagornykh MO — conceptualization, design of genetic constructs, article authoring; Ivanov RA — general management.
Received: 2023-11-16
Accepted: 2023-12-17
Published online: 2023-12-31
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