2023 / 06

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DOI: 10.24075/brsmu.2023.058

ORIGINAL RESEARCH

Preparation of a recombinant ribonuclease inhibitor in E. coli for use in mRNA synthesis in vitro

Zakharova MV1, Zagoskin AA1,2, Ivanov RA2, Nagornykh MO1,2
About authors

1 Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Russia

2 Sirius University of Science and Technology, Sirius, Sochi, Russia

Correspondence should be addressed: Maxim O. Nagornykh
Prospekt Nauki, 5, Pushchino, 142290, Russia; moc.liamg@rennabred

About paper

Funding: the study was financially supported in the context of the program of Ministry of higher education and science of the Russian Federation (agreement #075-10-2021-113, unique project number RF----193021X0001).

Author contribution: Zakharov MV — selection of conditions of production of recombinant proteins, production experiments in different E. coli strains; Zagoskin AA — chromatographic clearing of recombinant proteins; Nagornykh MO — conceptualization, design of genetic constructs, article authoring; Ivanov RA — general management.

Received: 2023-11-16 Accepted: 2023-12-17 Published online: 2023-12-31
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