2024 / 05

Next Paper
DOI: 10.24075/brsmu.2024.048
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Production and biological activity of the exogenous mRNA encoding human MxA protein

Plotnikova MA1, Bobkov DE1,2, Klotchenko SA1
About authors

1 Smorodintsev Research Institute of Influenza of the Ministry of Health of the Russian Federation, St. Petersburg, Russia

2 Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia

Correspondence should be addressed: Sergey A. Klotchenko
Professora Popova, 15/17, St. Petersburg, 197022, Russia; ur.liam@kitafsof

About paper

Funding: the study was supported by the Russian Science Foundation, Agreement No. 23-25-00433, project title: “Assessment of antiviral effect of the mRNA encoding human MxA protein” (manager M.A. Plotnikova), https://rscf.ru/project/23-25-00433/

Author contribution: Plotnikova MA — study design, experimental procedure, analysis of the results, statistical processing, manuscript writing; Bobkov DE — confocal microscopy examination; Klotchenko SA — production and characterization of the exogenous mRNA preparations, manuscript editing.

Received: 2024-09-30 Accepted: 2024-10-22 Published online: 2024-10-30
|
  1. Liao S, Gao S. MxA: a broadly acting effector of interferon-induced human innate immunity. Visualized Cancer Medicine. 2022; 3: 2. Available from: https://doi.org/10.1051/vcm/2022002.
  2. Verhelst J, Hulpiau P, Saelens X. Mx proteins: antiviral gatekeepers that restrain the uninvited. Microbiology and Molecular Biology Reviews. 2013; 77 (4): 551–66. Available from: https://doi.org/10.1128/mmbr.00024-13.
  3. Haller O, Kochs G. Interferon–induced mx proteins: dynamin–like GTPases with antiviral activity. Traffic. 2002; 3 (10): 710–7. Available from: https://doi.org/10.1034/j.1600-0854.2002.31003.x.
  4. Zürcher T, Pavlovic J, Staeheli P. Mechanism of human MxA protein action: variants with changed antiviral properties. The EMBO Journal. 1992; 11 (4): 1657–61. Available from: https://doi.org/10.1002/j.1460-2075.1992.tb05212.x.
  5. Johannes L, et al. Antiviral determinants of rat Mx GTPases map to the carboxy-terminal half. Journal of virology. 1997; 71 (12): 9792–5. Available from: https://doi.org/10.1128/jvi.71.12.9792-9795.1997.
  6. Jung HE, Oh JE, Lee HK. Cell-penetrating Mx1 enhances anti-viral resistance against mucosal influenza viral infection. Viruses. 2019; 11 (2): 109. Available from: https://doi.org/10.3390/v11020109.
  7. Sistigu A, et al. Cancer cell–autonomous contribution of type I interferon signaling to the efficacy of chemotherapy. Nature medicine. 2014; 20 (11): 1301–9. Available from: https://doi.org/10.1038/nm.3708.
  8. Kim YA, et al. MxA expression is associated with tumor-infiltrating lymphocytes and is a prognostic factor in triple-negative breast cancer. Breast cancer research and treatment. 2016; 156: 597–606. Available from: https://doi.org/10.1007/s10549-016-3786-z.
  9. Klotchenko S. A. et al. Comparative analysis of MxA, OAS1, PKR gene expression levels in leukocytes of patients with influenza and coronavirus infection. Medical academic journal. 2023; 23 (3): 65– 75. Available from: https://doi.org/10.17816/MAJ623374.
  10. Available from: https://www.ncbi.nlm.nih.gov/nuccore/.
  11. Noguchi S, et al. MxA transcripts with distinct first exons and modulation of gene expression levels by single-nucleotide polymorphisms in human bronchial epithelial cells. Immunogenetics. 2013; 65: 107–14. Available from: https://doi.org/10.1007/s00251-012-0663-8.
  12. Tazi-Ahnini R, et al. Structure and polymorphism of the human gene for the interferon-induced p78 protein (MX1): evidence of association with alopecia areata in the Down syndrome region. Human genetics. 2000; 106: 639–45. Available from: https://doi.org/10.1007/s004390000318.
  13. Elroy-Stein O, Fuerst TR, Moss B. Cap-independent translation of mRNA conferred by encephalomyocarditis virus 5'sequence improves the performance of the vaccinia virus/bacteriophage T7 hybrid expression system. Proceedings of the National Academy of Sciences. 1989; 86 16: 6126–30. Available from: https://doi.org/10.1073/pnas.86.16.6126.
  14. Bochkov YA, Palmenberg AC. Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location. Biotechniques. 2006; 41 (3): 283–92. Available from: https://doi.org/10.2144/000112243.
  15. Kariko K, Weissman D. Naturally occurring nucleoside modifications suppress the immunostimulatory activity of RNA: implication for therapeutic RNA development. Current Opinion in Drug Discovery and Development. 2007; 10 (5): 523. PMID: 17786850.
  16. Karikó K, et al. Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Molecular therapy. 2008; 16 11: 1833–40. Available from: https://doi.org/10.1038/mt.2008.200.
  17. Weissman D. mRNA transcript therapy. Expert review of vaccines. 2015; 14 (2): 265–81. Available from: https://doi.org/10.1586/14 760584.2015.973859.
  18. von Niessen AGO, et al. Improving mRNA-based therapeutic gene delivery by expression-augmenting 3′ UTRs identified by cellular library screening. Molecular Therapy. 2019; 27 (4): 824–36. Available from: https://doi.org/10.1016/j.ymthe.2018.12.011.
  19. Xue S, et al. RNA regulons in Hox 5′ UTRs confer ribosome specificity to gene regulation. Nature. 2015; 517 (7532): 33–38. Available from: https://doi.org/10.1038/nature14010.
  20. Koch A, et al. Quantifying the dynamics of IRES and cap translation with single-molecule resolution in live cells. Nature structural & molecular biology. 2020; 27 (12): 1095–104. Available from: https://doi.org/10.1038/s41594-020-0504-7.
  21. Haller O, Kochs G. Mx genes: host determinants controlling influenza virus infection and trans-species transmission. Human genetics. 2020; 139 (6): 695–705. Available from: https://doi.org/10.1007/s00439-019-02092-8.
  22. Kawai T, Akira S. The role of pattern-recognition receptors in innate immunity: update on Toll-like receptors. Nature immunology. 2010; 11 (5): 373–84. Available from: https://doi.org/10.1038/ni.1863.