ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Modified micro test tubes as a promising basis for immobilization of antibodies for immunocapture on the example of SARS-CoV-2
About authors
1 Astrakhan State Medical University, Astrakhan, Russia
2 Russian University of Medicine, Moscow, Russia
3 Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing, Moscow, Russia
4 Gamaleya National Research Centre for Epidemiology and Microbiology, Moscow, Russia
5 Lomonosov Moscow State University, Moscow, Russia
6 Sechenov First Moscow State Medical University, Moscow, Russia
Correspondence should be addressed: Evgeny O. Roubalsky
Bakinskaya, 121, Astrakhan, 414000, Russia; moc.liamg@ykslabur.o.e orp.egahp@ykslabur
About paper
Funding: the study was supported by the Russian Science Foundation grant No. 23-15-20035, https://rscf.ru/project/23-15-20035/
Author contribution: Rubalsky EO, Abdrakhmanova RA, Baeva GR, Rubalskaya TS — experimental procedure involving micro test tubes; Shcheblyakov DV, Favorskaya IA — preparation of monoclonal antibodies; Rubalsky EO, Lazko MV, Poroyskiy SV, Gushchin VA — study design. The authors contributed to the search for and analysis of information, preparation of the draft, and manuscript writing equally.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Astrakhan State Medical University. (protocol No. 7 dated 22 February 2023).
Received: 2024-11-29
Accepted: 2024-12-19
Published online: 2024-12-28
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Fig. 1. Polypropylene micro test tube after modification with polystyrene: side view (А) and ≈45° view (B)
Fig. 2. The GK2020/1 strain immunocapture results. The P2C5 monoclonal antibody is highlighted in red, R107 is highlighted in yellow, control group with BSA is highlighted in green. * * — p < 0.01; * * * — p < 0.001; * * * * — p < 0.0001
Fig. 3. The hCoV-19/Russia/MOW-PMVL-51/2021 strain immunocapture results. The P2C5 monoclonal antibody is highlighted in red, R107 is highlighted in yellow, control group with BSA is highlighted in green. * * * * — p < 0.0001
Fig. 4. Cross-contamination detected when extracting SARS-CoV-2 RNA in the 96-well plates. The wells depicted as red circles contain the GK2020/1 strain (variant B.1.1.1). The wells depicted as green circles contain the hCoV-19/Russia/MOW-PMVL-51/2021 strain (variant B.1.1.529+BA.*Omicron lineage). Red dots in green circles indicate contamination of the hCoV-19/Russia/MOW-PMVL-51/2021 strain with the GK2020/1 strain. Green dots in red circles indicate contamination of the GK2020/1 strain with the hCoV-19/Russia/MOW-PMVL-51/2021 strain. (А–C) Three sequential tests performed by three different operators
