ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Comparative analysis of the results of testing cervical epithelial samples and cervical biopsy specimens for HPV
About authors
Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia
Correspondence should be addressed: Gyuldana R. Bayramova
Akademika Oparina, 4B, Moscow, 117513, Russia; ur.liam@avomaryab
About paper
Funding: the study was conducted within the framework of the State Assignment No. 124040300006-2 “Development of a test system to detect early signs of cervical and endometrial cancer based on epigenetic DNA analysis in cervical smears”.
Acknowledgements: the authors would like to thank A.R. Zaretsky, head of the Laboratory of Molecular Oncogenetics, Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, for valuable comments on the study concept and analysis of the results.
Author contribution: Bayramova GR, Trofimov DYu — study concept and design; Andreev AO, Bourmenskaya OV — data acquisition and processing; Asaturova AV, Bourmenskaya OV — statistical data processing; Andreev AO, Piven VD — manuscript writing; Bayramova GR, Asaturova AV — manuscript editing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (protocol No. 11 dated 25 November 2021) and conducted in full compliance with the requirements for confidentiality of personal dara, ethical standards and principles of conducting medical studies involving humans stated in the Declaration of Helsinki of the World Medical Association “Ethical principles for medical research involving human subjects”.
Received: 2024-12-13
Accepted: 2024-12-26
Published online: 2024-12-31
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Currently, testing for human papillomavirus (HPV) DNA is more and more often used as a primary diagnosis method when conducting screening for cervical cancer. However, HPV genotypes reported when assessing cervical smears can differ from the results of testing cervical biopsy specimens. The study aimed to assess the features of detecting HPV DNA in the paired cervical canal epithelium samples and cervical biopsy specimens. HPV-positive patients (n = 99) underwent targeted cervical biopsy. The HPV DNA was detected 175 times in biomaterial obtained from the cervical canal and 111 times in histologic blocks. In the group of patients with chronic cervicitis, the rate of HPV DNA testing results match was 28.3%, in the group with LSIL it was 45%, and in the group with HSIL it was 67.7%. When the HPV viral load was low, the results were matched in 27.1% of cases, when the viral load was moderate in 35.4%, and when the viral load was high these were matched in 82.3% of cases. We revealed a relatively strong correlation between the viral load and the probability of the HPV test results match: the percentage of HPV DNA test results match between paired samples increases by 9.3% with the increase in the HPV viral load by 1 lg.
Keywords: HPV testing, HPV viral load, cervical pathology, cervical biopsy