ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
The effect of sterilization methods on the cytotoxicity of ceramic medical implants
About authors
Bashkir State Medical University of the Ministry of Health of the Russian Federation, Ufa, Russia
Correspondence should be addressed: Azat R. Bilyalov
Lenina, 3, ap. 119, 450008, Ufa, Republic of Bashkortostan, Russia; moc.liamg@volaylib.taza
About paper
Funding: the work was supported by the Russian Science Foundation under grant No. 23-15-20042.
Author contribution: Bilyalov AR — stuidy conceptualization, data analysis, article editing; Piatnitskaia SV — cytotoxicity evaluation (MTT test), analysis of the results; Rafikova GA — preparation of digital models and sample production, analysis of mechanical and biological properties of materials; Akbashev VN — sterilization experiments, analysis of the effect of sterilization methods on materials; Bikmeyev AT — mathematical modeling of material parameters, interpretation of the data obtained; Akhatov IS — coordination of work, general guidance, article editing; Shangina OR — analysis of the samples' porosity and density, statistical data processing; Chugunov SS — samples heat treatment and sintering, description of materials and methods; Tikhonov AA — assessment of microstructural changes using SEM, writing the section "Electron microscopy."
Received: 2024-12-14
Accepted: 2025-02-18
Published online: 2025-02-27
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Fig. 1. Cylindrical 3D model of ceramic samples, 20% shrinkage factored in
Fig. 2. Printing samples of hydroxyapatite on a Ceramaker 900 3D printer
Fig. 3. 3D printed ceramic samples after mechanical cleaning
Fig. 4. Ceramic samples from HA and TCP after annealing and sintering
Fig. 5. Printing of ceramic samples from a bone allograft in an Elegoo Mars 4 3D printer
Fig. 6. Ceramic samples from bone allograft after annealing and sintering
Fig. 7. The microstructure of the additive material made from hydroxyapatite (A1–3), tricalcium phosphate (B1–3), aluminum oxide (C1–3)
Fig. 8. The dependence of cell survival in the studied samples on the degree of porosity when subjected to various sterilization methods
Table. MTT test results for the studied materials with different porosities
