ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Characteristics of the metastasis-associated circulating cells: features of side scatter parameters
About authors
1 Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia
2 Siberian State Medical University, Tomsk, Russia
3 Saint Petersburg State Pediatric Medical University, Saint Petersburg, Russia
Correspondence should be addressed: Angelina V. Buzenkova
Kooperativny per., 5, Tomsk, 634009, Russia; ur.liam@va_avoknezub
About paper
Funding: the study was supported by the RSF grant No. № 23-15-00135.
Author contribution: Buzenkova AV — literature review, data analysis, acquisition and statistical processing of the results, manuscript writing; Grigoryeva ES — flow cytometry, data analysis, interpretation of the results, manuscript writing; Alifanov VV — flow cytometry, manuscript editing; Tashireva LA, Savelieva OE — discussion, manuscript editing; Pudova ES — flow cytometry; Zavyalova MV — manuscript editing; Cherdyntseva NV — study planning and design, discussion; Perelmuter VM — study planning and management, interpretation of the results, manuscript writing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences (protocol No. 8 dated 17 June 2016) and conducted in accordance with Federal Laws of the Russian Federation (No. 152, 323, etc.), the Declaration of Helsinki (1964) and all later amendments and additions that regulate scientific research involving human biomaterial. All subjects submitted the informed consent to participation in the study.
Received: 2025-03-11
Accepted: 2025-03-26
Published online: 2025-04-18
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Fig. 1. Distribution of CCs (CD45– mEpCAM–CK7/8–CD24+Ncadh–) within the SSClow and SSChigh regions (cells of interest are highlighted in red)
Fig. 2. Comparison of the abundance and counts in blood of donors and patients with IDC NST (main phenotype: CD45– mEpCAM– CK7/8– CD24+Ncadh– — with co-expression of CD11b, E-cadherin, pan-Cytokeratin и icEpCAM) within the SSClow and SSChigh regions. A. Comparison of CC abundance in donors and patients within the SSClowregion. B. Comparison of CC abundance in donors and patients within the SSChigh region. C. Comparison of CC counts in donors and patients within the SSClow region. D. Comparison of CC counts in donors and patients within the SSChigh region
Fig. 3. Total abundance and counts of the CD11b– and CD11b+ cell that express any epithelial cell marker (main phenotype: CD45– mEpCAM– CK7/8– CD24+Ncadh– , epithelial cell markers: E-cadh, panCK, icEpCAM) within the SSClow and SSChigh regions. A. Comparison of the total abundance of the CC epithelial markers in donors and patients with IDC NST within the SSClow and SSChigh regions. B. Comparison of total counts of the CC epithelial markers in donors and patients with IDC NST within the SSClow and SSChigh regions
Table 1. Comparison of CC abundance within the SSClow and SSChigh regions in patients with IDC NST
Table 2. Comparison of CC counts within the SSClow and SSChigh regions in patients with IDC NST
Table 3. Comparison of CC abundance and counts within the SSClow and SSChigh regions in donors
