2025 / 02

Next Paper
DOI: 10.24075/brsmu.2025.020
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Determination of the rate of autoantibody carrier state in patients with celiac disease by mono- and multiplex immunoassay

Nuralieva NF1, Yukina MYu1, Bykova SV2, Savvateeva EN3, Nikankina LV1, Kulagina EV3, Shaskolskiy BL3, Gryadunov DA3, Troshina EA1
About authors

1 Endocrinology Research Centre, Moscow, Russia

2 Loginov Moscow Clinical Scientific Centre, Moscow, Russia

3 Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Moscow

Correspondence should be addressed: Nurana F. Nuralieva
Dmitriya Ulyanova, 11, Moscow, 117292, Russia; ur.xednay@anarunn

About paper

Funding: the study was supported by the Foundation for Scientific and Technological Development of Yugra (No. 2023-571-05/2023).

Author contribution: Nuralieva NF — literature review, study concept and design, patient assessment, material collection, laboratory testing, analysis and interpretation of the results, manuscript writing; Yukina MYu — literature review, study concept and design, patient assessment, material collection, laboratory testing, analysis and interpretation of the results, manuscript writing and editing; Bykova SV — patient assessment, material collection, manuscript editing; Savvateeva EN — literature review, study concept and design; laboratory microarray testing; analysis and interpretation of the results; Kulagina EV, Nikankina LV — laboratory testing (ELISA); Shaskolskiy BL — analysis of the autoantibody multiplex testing results; Gryadunov DA — study concept and design; manuscript editing; Troshina EA — manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Endocrinology Research Centre (protocol No. 14 dated 29 July 2022). All the patients and conditionally healthy individuals submitted the informed consent to participation in the study.

Received: 2025-04-02 Accepted: 2025-04-16 Published online: 2025-04-23
|
  1. Ahmedova JeF, Belinskaja VA. Gnezdnaja alopecija kak osnovnoe projavlenie celiakii. Jeksperimental'naja i klinicheskaja gastrojenterologija. 2022; 205 (9): 303–07. DOI: 10.31146/1682-8658-ecg-205-9-303-307. Russian.
  2. Bykova SV, Sabel'nikova EA, Zadiran EI, Parfenov AI. Osvedomlennost' vrachej o celiakii: rezul'taty oprosa. Jeffektivnaja farmakoterapija. 2021; 17 (16): 92–100. DOI: 10.33978/2307-3586-2021-17-16-92-100. Russian.
  3. Parfenov AI. Celiakija (bolezn' Gi–Gertera–Gejbnera). M.: Medicinskoe informacionnoe agentstvo, 2023; 488 c. Russian.
  4. Parfenov AI, Bykova SV, Sabel'nikova EA, Maev IV, Baranov AA, Bakulin IG, i dr. Vserossijskij konsensus po diagnostike i lecheniju celiakii u detej i vzroslyh. Terapevticheskij arhiv. 2017; 89 (3): 94– 107. DOI: 10.17116/terarkh201789394-107. Russian.
  5. Kocsis D, Csaplár M, Jócsák E, Pák P, Tóth Z, Miheller P, et al. Celiac disease association with other autoimmune disorders: Three case reports. Case Reports in Internal Medicine. 2015; 2 (1): 23–29. DOI: 0.5430/crim.v2n1p23.
  6. Lauret E, Rodrigo L. Celiac disease and autoimmune-associated conditions. Biomed Research International. 2013; 2013: 127589. DOI: 10.1155/2013/127589.
  7. Caglar E, Ugurlu S, Ozenoglu A, Can G, Kadioglu P, Dobrucali A. Autoantibody frequency in celiac disease. Clinics. 2009; 64 (12): 1195–200. DOI: 10.1590/S1807-59322009001200009.
  8. Lochman I, Martis P, Burlingame RW, Lochmanová A. Multiplex assays to diagnose celiac disease. Annals of the New York Academy of Sciences. 2007; 1109: 330–7. DOI: 10.1196/annals.1398.039.
  9. Holding S, Wilson F, Spradbery D. Clinical evaluation of the BioPlex 2200 Celiac IgA and IgG Kits — a novel multiplex screen incorporating an integral check for IgA deficiency. Journal of Immunological Methods. 2014; 405: 29–34. DOI: 10.1016/j.jim.2014.01.002.
  10. Abdukhakimova D, Dossybayeva K, Grechka A, Almukhamedova Z, Boltanova A, Kozina L, et al. Reliability of the multiplex CytoBead CeliAK immunoassay to assess Anti-tTG IgA for celiac disease screening. Frontiers in Medicine. 2021; 8: 731067. DOI: 10.3389/fmed.2021.731067.
  11. Gu Y, Zhao Z, Waugh K, Miao D, Jia X, Cheng J, et al. High-throughput multiplexed autoantibody detection to screen type 1 diabetes and multiple autoimmune diseases simultaneously. EBioMedicine. 2019; 47: 365–72. DOI: 10.1016/j.ebiom.2019.08.036.
  12. Savvateeva EN, Yukina MY, Nuralieva NF, Filippova MA, Gryadunov DA, Troshina EA. Multiplex autoantibody detection in patients with autoimmune polyglandular syndromes. International Journal of Molecular Sciences. 2021; 22 (11): 5502. DOI: 10.3390/ijms22115502.
  13. Rashid M, Lee J. Serologic testing in celiac disease: Practical guide for clinicians. Canadian Family Physician. 2016; 62 (1): 38–43.
  14. Odineal DD, Gershwin ME. The epidemiology and clinical manifestations of autoimmunity in selective IgA deficiency. Clinical Reviews in Allergy & Immunology. 2020; 58 (1): 107–33. DOI:10.1007/s12016-019-08756-7.
  15. Gupta V, Singh A, Makharia GK. Prevalence of auto-antibodies and autoimmune disorders in patients with celiac disease. Scandinavian Journal of Gastroenterology. 2020; 55 (7): 785. DOI: 10.1080/00365521.2020.1785544.
  16. Volta U, De Franceschi L, Molinaro N, Tetta C, Bianchi FB. Organ-specific autoantibodies in coeliac disease: do they represent an epiphenomenon or the expression of associated autoimmune disorders? Italian Journal of Gastroenterology and Hepatology. 1997; 29: 18–21.
  17. Patel SR, Shashaty RJ, Denoux P. Nutritional Nightmare: Hypoparathyroidism Secondary to Celiac Disease. The American Journal of Medicine. 2017; 130 (12): e525–e526. DOI: 10.1016/j.amjmed.2017.07.032.
  18. Ghozzi M, Souguir D, Melayah S, Abidi S, Faleh M, Ghedira I. Frequency of auto-antibodies of type 1 diabetes in adult patients with celiac disease. Journal of Clinical Laboratory Analysis. 2021; 35 (9): e23941. DOI: 10.1002/jcla.23941.
  19. Tiberti C, Panimolle F, Borghini R, Montuori M, Trovato CM, Filardi T, et al. Type 1 diabetes, thyroid, gastric and adrenal humoral autoantibodies are present altogether in almost one third of adult celiac patients at diagnosis, with a higher frequency than children and adolescent celiac patients. Scandinavian Journal of Gastroenterology. 2020; 55 (5): 549–54. DOI: 10.1080/00365521.2020.1754898.
  20. Ventura A, Neri E, Ughi C, Leopaldi A, Citta A, Not T. Glutendependent diabetes-related and thyroid-related autoantibodies in patients with celiac disease. The Journal of Pediatrics. 2000; 137: 263–65. DOI: 10.1067/mpd.2000.107160.
  21. Velluzzi F, Caradonna A, Boy MF, Pinna MA, Cabula R, Lai MA, et al. Thyroid and celiac disease: clinical, serological, and echographic study. American Journal of Gastroenterology. 1998; 93: 976–979. DOI: 10.1111/j.1572-0241.1998.291_u.x.
  22. Carta MG, Hardoy MC, Boi MF, Mariotti S, Carpiniello B, Usai P. Association between panic disorder, major depressive disorder and celiac disease: a possible role of thyroid autoimmunity. Journal of Psychosomatic Research. 2002; 53: 789–93. DOI: 10.1016/s0022-3999(02)00328-8.
  23. Gaibi S, Mohammad Amini H, Feizollah Zadeh S. Investigating the prevalence of autoimmune thyroid diseases in children with celiac disease in Urmia city, Iran. Studies in Medical Sciences. 2023; 34(6): 330–37.
  24. Hadjivassiliou M, Aeschlimann D, Grünewald RA, Sanders DS, Sharrack B, Woodroofe N. GAD antibody-associated neurological illness and its relationship to gluten sensitivity. Acta Neurologica Scandinavica. 2011; 123 (3): 175–80. DOI: 10.1111/j.1600-0404.2010.01356.x.
  25. Gupta S, Tatouli IP, Rosen LB, Hasni S, Alevizos I, Manna ZG, et al. Distinct functions of autoantibodies against interferon in systemic lupus erythematosus: a comprehensive analysis of anticytokine autoantibodies in common rheumatic diseases. Arthritis & Rheumatology. 2016; 68 (7): 1677–87. DOI: 10.1002/art.39607.
  26. Savvateeva E, Filippova M, Valuev-Elliston V, Nuralieva N, Yukina M, Troshina E, et al. Microarray-based detection of antibodies against SARS-CoV-2 proteins, common respiratory viruses and type I interferons. Viruses. 2021; 13: 2553. DOI: 10.3390/v13122553.
  27. Zimmerman RK, Balasubramani GK, Nowalk MP, Eng H, Urbanski L, Jackson M L, et al. Classification and Regression Tree (CART) analysis to predict influenza in primary care patients. BMC Infectious Diseases. 2016; 16 (1): 503. DOI: 10.1186/s12879-016-1839-x.
  28. Sarna VK, Lundin KEA, Mørkrid L, Qiao SW, Sollid LM, Christophersen A. HLA-DQ-gluten tetramer blood test accurately identifies patients with and without celiac disease in absence of gluten consumption. Gastroenterology. 2018; 154 (4): 886–896. e6. DOI: 10.1053/j.gastro.2017.11.006.
  29. Elia ZN, Berwary NJA. Effectiveness of a gluten-free diet on the autoantibody frequency of endocrine and neuron disorders in patients with celiac disease. Journal of Advanced Biotechnology and Experimental Therapeutics. 2024; 7 (1): 34–43. DOI: 10.5455/jabet.2024.d03.
  30. Tiberti C, Montuori M, Trovato CM, Panimolle F, Filardi T, Valitutti F, et al. Gluten-free diet impact on dynamics of pancreatic islet-specific autoimmunity detected at celiac disease diagnosis. Pediatric Diabetes. 2020; 21 (5): 774–80. DOI: 10.1111/pedi.13054.