ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Determination of aging phenotype based on the changes in spontaneous and induced interleukin-6 and interleukin-10 production in vitro
About authors
Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Tatiana V. Gromova
Ostrovityanova, 1, stroenie 9, Moscow, 117997; moc.liamg@avohsavelvt
About paper
Funding: the study was supported by the Russian Science Foundation grant No. 23-15-00137, https://rscf.ru/project/23-15-00137.
Acknowledgements: to O.N. Tkacheva, Head of the Russian Gerontological Research and Clinical Center, Pirogov Russian National Research Medical University, for the provided biomaterial samples of the group of centenarians; executives of the Diagnostic Clinical Center No. 1, Obruchevsky Social House, branch of the Troparyovo Geriatric Center, for the provided biomaterial samples of patients of older age groups.
Author contribution: Gankovskaya LV — research management and study planning; Grechenko VV, Gromova TV, Ogurtsova AD, Zhuravleva ER — data acquisition, processing, and analysis; Grechenko VV — statistical processing, Muradyan TG — selection of clinical groups; Grechenko VV, Gromova TV, Gankovskaya LV — manuscript writing; Ogurtsova AD — editing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Pirogov Russian National Research Medical University (protocol No. 214 dated 13 December 2021). The subjects submitted the informed consent to participation in the study and the consent to data processing.
Received: 2025-08-03
Accepted: 2025-08-20
Published online: 2025-08-30
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Fig. 1. Spontaneous IL6 (А) and IL10 (B) production in the culture of peripheral blood MNCs of elderly individuals, senile individuals, and centenarians with different aging phenotypes. The data are presented as the median (Me) and interquartile range, significant differences between aging phenotypes within the studied age groups: * — p < 0.05; * * — p < 0.01
Fig. 2. LPS-induced IL6 (А) and IL10 (B) production in the culture of peripheral blood MNCs of elderly individuals, senile individuals, and centenarians with different aging phenotypes. The data are presented as the median (Me) and interquartile range, significant differences between aging phenotypes within the studied age groups: * — p < 0.05; * * — p < 0.01; * * * — p < 0.001
Fig. 3. Stimulation coefficients for IL6 (А) and IL10 (B) production in the culture of peripheral blood MNCs of elderly individuals, senile individuals, and centenarians with different aging phenotypes. The data are presented as the median (Me) and interquartile range, significant differences between aging phenotypes: * — p < 0.05; * * — p < 0.01; * * * — p < 0.001; significant differences relative to the comparison group (young donors): # — p < 0.05; ## — p < 0.01; ### — p < 0.001
Table. Criteria for dividing the studied groups into subgroups with successful and pathological aging
Note: * — based on the data [7].
