2025 / 04

DOI: 10.24075/brsmu.2025.041
Copyright: © 2025 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Determination of aging phenotype based on the changes in spontaneous and induced interleukin-6 and interleukin-10 production in vitro

Grechenko VV, Gromova TV, Ogurtsova AD, Muradyan TG, Zhuravleva ER, Gankovskaya LV
About authors

Pirogov Russian National Research Medical University, Moscow, Russia

Correspondence should be addressed: Tatiana V. Gromova
Ostrovityanova, 1, stroenie 9, Moscow, 117997; moc.liamg@avohsavelvt

About paper

Funding: the study was supported by the Russian Science Foundation grant No. 23-15-00137, https://rscf.ru/project/23-15-00137.

Acknowledgements: to O.N. Tkacheva, Head of the Russian Gerontological Research and Clinical Center, Pirogov Russian National Research Medical University, for the provided biomaterial samples of the group of centenarians; executives of the Diagnostic Clinical Center No. 1, Obruchevsky Social House, branch of the Troparyovo Geriatric Center, for the provided biomaterial samples of patients of older age groups.

Author contribution: Gankovskaya LV — research management and study planning; Grechenko VV, Gromova TV, Ogurtsova AD, Zhuravleva ER — data acquisition, processing, and analysis; Grechenko VV — statistical processing, Muradyan TG — selection of clinical groups; Grechenko VV, Gromova TV, Gankovskaya LV — manuscript writing; Ogurtsova AD — editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Pirogov Russian National Research Medical University (protocol No. 214 dated 13 December 2021). The subjects submitted the informed consent to participation in the study and the consent to data processing.

Received: 2025-08-03 Accepted: 2025-08-20 Published online: 2025-08-30
|
  1. Franceschi C, Bonafe M, Valensin S, Olivieri F, De Luca M, Ottaviani E, et al. Inflamm-aging. An evolutionary perspective on immunosenescence. Ann N Y Acad Sci. 2000; 908: 208–18. Available from: https://doi.org/10.1111/j.1749-6632.2000.tb06651.x.
  2. Gankovskaya LV, Artemyeva OV, Namazova-Baranova LS, et al. Immunologicheskie aspekty starenija i vozrast-associirovannaja patologija. M.: Pediatr, 2021; 156 s. Russian.
  3. Franceschi C, Capri M, Monti D, et al. Inflammaging and anti-nflammaging: a systemic perspective on aging and longevity emerged from studies in humans. Mechanisms of Ageing and Development. 2007; 128: 92–105. DOI: 10.1016/j.mad.2006.11.016.
  4. Minciullo PL, Catalano A, Mandraffino G, Casciaro M, Crucitti A, Maltese G, et al. Inflammaging and Anti-Inflammaging: The Role of Cytokines in Extreme Longevity. Arch Immunol Ther Exp. 2016; 64: 111–26. DOI: 10.1007/s00005-015-0377-3.
  5. Puzianowska-Kuznicka M, Owczarz M, Wieczorowska-Tobis K, Nadrowski P, Chudek J, et al. Interleukin-6 and C-reactive protein, successful aging, and mortality: the PolSenior study. Immun Aging. 2016; 13: no.21. DOI: 10.1186/s12979-016-0076-x.
  6. Grechenko VV, Artemyeva OV, Gromova TV, Gankovskaya LV. Izmenenie urovnej pro- i protivovospalitel'nyh citokinov u lic starcheskogo vozrasta i dolgozhitelej pri razlichnyh fenotipah starenija. Immunologija. 2024; 45 (5): 594–603. Available from: https://doi.org/10.33029/1816-2134-2024-45-5-594-603. Russian. Available from: https://doi.org/10.33029/1816-2134-2024-45-5-594-603.
  7. Hayashida DY, Jacinto AF, Araújo LMQ, Almada Filho CMDI, Tommaso AB, Cendoroglo MS. Association between baseline Mini-Mental State Examination score and dementia incidence in a cohort of oldest old. Arq Neuropsiquiatr. 2021; 79 (12): 1090–4. DOI: 10.1590/0004-282X-ANP-2020-0543.
  8. Zhu W, Revu S, Chen C, Dahl M, Ramkumar A, Kelly C, et al. Aging-dependent change in Th17 and cytokine response in multiple sclerosis. J Neuroinflammation. 2025; 22 (1): 150. DOI: 10.1186/s12974-025-03474-8. PMID: 40474243; PMCID: PMC12142853.
  9. Bogatyreva AI, Gerasimova EV, Kirichenko TV, Markina YV, Popkova TV, Shalygina MV, et al. Proinflammatory Activation of Monocytes in Patients with Immunoinflammatory Rheumatic Diseases. Dokl Biochem Biophys. 2024; 517 (1): 228–34. DOI: 10.1134/S1607672924700959. Epub 2024 Jul 13. PMID: 39002011.
  10. Franceschi C, Salvioli S, Garagnani P, de Eguileor M, Monti D, Capri M. Immunobiography and the heterogeneity of immune responses in the elderly: a focus on inflammaging and trained immunity. Front in Immunology. 2017; 8: 982. Available from: https://doi.org/10.3389/fimmu.2017.00982.
  11. Pinti M, et al. A Comprehensive Analysis of Cytokine Network in Centenarians. Int J Mol Sci. 2023; 24 (3): 2719. DOI: 10.3390/ijms24032719.
  12. Tylutka A, Walas L, Zembron-Lacny A. Level of IL-6, TNF, and IL-1b and age-related diseases: a systematic review and meta-analysis. Front Immunol. 2024; 15: 1330386. DOI: 10.3389/fimmu.2024.1330386.
  13. Arosio B, Ferri E, Mari D, Tobaldini E, Vitale G, Montano N. The influence of inflammation and frailty in the aging continuum. Mech Ageing Dev. 2023; 215:111872. DOI: 10.1016/j.mad.2023.111872).
  14. Franceschi C. Inflammaging as a major characteristic of old people: can it be prevented or cured. Nutr Rev. 2007; 65 (12 Pt 2): 173–76. DOI: 10.1111/j.1753-4887.2007.tb00358.x.
  15. Zhou L, Ge M, Zhang Y, Wu X, Leng M, Gan C, et al. Centenarians Alleviate Inflammaging by Changing the Ratio and Secretory Phenotypes of T Helper 17 and Regulatory T Cells. Front Pharmacol. 2022; 13: 877709. DOI: 10.3389/fphar.2022.877709.
  16. Fulop T, Larbi A, Pawelec G, Khalil A, Cohen AA, Hirokawa K, et al.. Immunology of Aging: the Birth of Inflammaging. Clinical Reviews in Allergy & Immunology. 2023; 64: 109–22. DOI: https://doi.org/10.1007/s12016-021-08899-6.
  17. Bailey KL, Smith LM, Heires AJ, Katafiasz DM, Romberger DJ, LeVan TD. Aging leads to dysfunctional innate immune responses to TLR2 and TLR4 agonists. Aging Clin Exp Res. 2019; 31 (9): 1185–93. DOI: 10.1007/s40520-018-1064-0).
  18. Olivieri F, Prattichizzo F, Lattanzio F, Bonfigli AR, Spazzafumo L. Antifragility and antiinflammaging: Can they play a role for a healthy longevity? Ageing Research Reviews. 2023; 84: 101836. DOI: 101836https://doi.org/10.1016/j.arr.2022.101836.
  19. Dimitrijević M, Aleksić I, Vujić V, Stanojević S, Pilipović I, von Hörsten S, et al. Peritoneal exudate cells from long-lived rats exhibit increased IL-10/IL-1β expression ratio and preserved NO/ urea ratio following LPS-stimulation in vitro. Age (Dordr). 2014; 36 (4): 9696. DOI: 10.1007/s11357-014-9696-2).
  20. Silva R, Travassos L, Dutra F. The dichotomic role of single cytokines: Fine-tuning immune responses. Cytokine. 2024; 156408. DOI: https://doi.org/10.1016/j.cyto.2023.156408.
  21. Silva R. The dichotomic role of cytokines in aging. Biogerontology. 2025; 26: 17. Available from: https://doi.org/10.1007/s10522-024-10152-4.