Funding: the work was carried out under the research project "Cytoprotective Effects of Inert Gases for the Prevention and Treatment of Organ Dysfunction in Critical Conditions" (No. FGWS-2025-0015).

Author contribution: Dementev IM — conducting the model experiment, analysis and discussion of results, manuscript writing; Gabitov MV — analysis of results, statistical analysis, manuscript writing and editing; Timoshin SS — discussion of results and manuscript editing; Kuzovlev AN — study planning, discussion of results, manuscript editing; Grebenchikov OA — approval of the study design, discussion of results, manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology (Protocol No. 2/25/5 dated March 26, 2025). All animal procedures were performed in accordance with the principles of the European Convention for the Protection of Vertebrate Animals used for Experimental and Other Scientific Purposes (Strasbourg, 1986). The report of the study was prepared in accordance with the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines.