Method New Approaches for Screening of Cardiac Troponin I in Plasma
Agafonova LE, Shumkov AA, Shumyantseva VV, Archakov AI
The aim of the study was to develop new approaches for rapid and sensitive screening of cardiac troponin I in plasma. In this paper we developed a gravimetric and electrochemical immunosensors with experimental detection limits of cardiac troponin I in plasma: 0.4 ng/mL (17 pM) and 0.1 ng/mL (4 pM), which corresponds to the physiological level of cardiac marker in plasma for the diagnosis of acute myocardial infarction. The kinetics of highly specific interactions between the monoclonal antibody of cardiac troponin I and cardiac troponin I were also investigated by quartz crystal microbalance in real time, without multiple labeling procedures and multi-step process of immobilization of antibodies on a gold electrode, and secondary antibody staining.
Received: 2013-03-22
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3213
History of medicine Department of Traumatology, Orthopedics and Battle-Field Surgery of N.I.Pirogov RNRMU Is 80 Years Old
Kopenkin SS, Skoroglyadov AV
The article is devoted to the history of the Department of Traumatology, Orthopedics and Battle-Field Surgery of N.I.Pirogov RNRMU. There were described the basic directions of scientific, medical, educational activities of the team for 80 years, and the results achieved. The prospects of the Department's work under modern conditions are considered. 
Received: 2013-06-17
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3317
Miscellaneous Test Control — an Innovative Approach to the Assessment of Students’ Knowledge
Polunina NV, Levchuk IP, Shimanovskii NL, Ashanina NM, Kurochka AV
In an innovative education the most effective means of controlling the assimilation of theoretical knowledge is a computer testing. By participating in the production of accrediting teaching measurement materials as part of the competition announced by National Accreditation Agency in the Field of Education, the departments of public health and healthcare and emergency medicine of RNRMU named after N. I. Pirogov mastered the technique of the development of test items for the test control in their disciplines. The methodology includes two main parts: the preparation of the content structure of the discipline and the development of the standardized test items. There should be ensured stylistic requirements and pedagogical equivalence of tasks. Prospects for the development of innovative forms of the educational process are largely dependent on the degree of use of computer-based testing, including a remote one.
Received: 2012-10-09
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3417
Original research Age Dynamics of Muscle Strength in Modern Schoolchildren
Milushkina OYu, Fedotov DM, Bokareva NA, Skoblina NA
The study of the functional capacities of today's schoolchildren' organisms in relation to the same parameters of the same age schoolchildren of the 60's and 80's of the twentieth century was carried out on the basis of the analysis of changes in hand muscles strength. The findings show that functional capacities of modern children, both boys and girls, are reduced. It is particularly important in the analysis of the long-term dynamics of the values of hand strength, which is one of the integral parameters of a child's body. The causes of the decline of carpal dynamometry are: low physical activity, migration. The results obtained can be used for the objective evaluation of functional capacities in modern schoolchildren aged 8–15 years.
Received: 2012-10-29
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3853
Original research The Relationship between Hormonal Status and Ergo-Spirometry Data in Winter Sports Athletes
Nikitina LYu, Soodaeva SK, Shashkova TV, Petrovskaya YuA, Ulyanov AA, Gasymova SSh, Giryatovich EG, Ugorelova EA, Timchenko AYu, Petrovskiy FI, Chuchalin AG
The aim of the study was to estimate the interconnection between cardiopulmonary exercise testing data and hormonal status of athletes. Adolescent and young adult winter sports athletes (n = 86), including biathlonists, skiers, ice-hockey players, snowboarders with middle age of 20,8 years old were examined. The cardiopulmonary exercise testing (MasterScreen Pneumo, Jaeger, Germany) was performed in a traditional 8-minute treadmill-test. Thyroid stimulating hormone, free thyroxin, cortisol, testosterone were examined in venous blood samples. The cardiopulmonary testing data, which characterize fitness, were significantly higher in males. In athletes, who did not reach the anaerobic threshold (AT), testosterone level was twice higher, than in those with estimated AT. The results of multiple stepwise regression analysis showed, that testosterone was the only one index, which influenced on the ergo-spirometry data distribution and associated with exercise performance in athletes.
Received: 2012-05-21
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3571
Original research Intracellular pH as the Early Differential Marker of Glucocorticoid-Induced Apoptosis of Skin Fibroblasts
Dukhanin AS, Malkin PA, Shimanovskii NL
Early stages of dexamethasone-induced fibroplast apoptosis include changes in intracellular homeostasis determined by decreasing pH of the cytoplasm and increased intracellular calcium ion concentration. Na<sup>+</sup>/H<sup>+</sup>-transporter and plasma membrane calcium channels are molecular targets for the action of dexamethasone.
Received: 2012-07-16
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3785
Original research The State of Microvascular Stream and Water Metabolism of Denervated Liver in the Case of Tetrachloromethane Intoxication
Tsibulevsky AYu, Dubovaya TK, Schegolev AI, Oettinger AP, Poskrebysheva EA, Baranich TI
The purpose of the work was studying of the autonomous nervous system's role in regulations of a liver's reactivity. Acute intoxication with carbon tetrachloride (CTC) in rats on the background of vagotomy served as a model. In those conditions the liver's reactive properties were investigated on the example of microvascular stream's and water metabolism's reorganization. With the help of morphological and biophysical methods it was revealed that CTC intoxication was accompanied by disturbances of liver's water metabolism which manifested in increasing of organ's total water saturation and decreasing of its structure extent. Morphological basis of those disturbances was dilatation of sinusoids and blood stasis in them, as well as hepatocytes' specific volume increasing. It was established, that the response of the liver to CTC toxicity in vagotomised rats was characterized by the lower degree of severity of changes in the water exchange in comparison with animals which were not performed vagotomy. That intensity was less in vagotomised rats' modification than in the initially intact animals. Peculiarities of the organ's structural reorganizations lay in the absence of deviations of such parameters as perimeter of sinusoids and nuclear-plazma ratio.
Received: 2012-07-06
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3597
Original research The Role of Medical Activity of Family in Formation of the Health of Children with Respiratory Diseases
Polunina NV, Polunina VV, Avsazhanishvili VN
Decrease of child population, the rise in the incidence in general, including respiratory diseases, indicate the need for the detailed study of peculiarities of children's morbidity in this group and factors conditioning the formation of adverse health outcomes. There were examined 1257 children, analyzed the level and structure of their diseases, there were studied the main characteristics of the medical activity of parents and children. That allowed to identify the most important risk factors of morbidity. The resulting data formed the basis for the development of measures to improve the health of children with adverse health outcomes. The implementation of the recommendations provided an opportunity to positively change medical behavior of patents and improve the health of children.
Received: 2012-10-09
Published online: 2017-01-05
DOI:
Comments 0
VIEWS 3615