2020 / 04

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DOI: 10.24075/brsmu.2020.049

ORIGINAL RESEARCH

Hypoxia enhances transcytosis in intestinal enterocytes

Maltseva DV1, Shkurnikov MYu1,2, Nersisyan SA1, Nikulin SV1, Kurnosov AA1, Raigorodskaya MP3, Osipyants AI2,4, Tonevitsky EA5
About authors

1 National Research University Higher School of Economics, Moscow, Russia

2 P. A. Hertsen Moscow Oncology Research Center, branch of the National Medical Research Radiology Center, Moscow, Russia

3 SRC Bioclinicum, Moscow, Russia

4 Far Eastern Federal University, Vladivostok, Russia

5 Fund for Development of Innovative Scientific-Technological Center Mendeleev Valley, Moscow, Russia

Correspondence should be addressed: Diana V. Maltseva
Vavilova, 7, Moscow, 117321; moc.liamg@avestlamd

About paper

Funding: the study was supported by the Ministry of Science and Higher Education of the Russian Federation (Project ID RFMEFI61719X0056).

Acknowledgement: the authors thank the Human Proteome Core Facility (Institute of Biomedical Chemistry) for permission to use the Facility’s equipment.

Author contribution: Maltseva DV — molecular tests, analysis of their results, manuscript preparation; Shkurnikov MYu — analysis of transcriptome and sequencing data, statistical analysis; Nersisyan SA — sequencing data processing, bioinformatic analysis, functional gene analysis; Nikulin SV — cell culture, sample preparation for subsequent proteome analysis, proteomic data analysis; Kurnosov AA — sample preparation for microRNA sequencing, analysis of sequencing data; Raigorodskaya MP — real-time PCR-based analysis of gene expression, transcriptome analysis; Osipyants AI — cell culture, sample preparation for subsequent proteome and transcriptome analyses; Tonevitsky EA — supervision, data analysis, manuscript preparation.

Received: 2020-08-08 Accepted: 2020-08-21 Published online: 2020-08-28
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Fig. 1. Transepithelial electrical resistance (TEER) at different time points following the treatment of enterocytes with ricin
Fig. 2. The amount of biotinylated lectin transcytosed across enterocytes under normoxic conditions (control) and in the hypoxic environment; * — lectin proportion transcytosed after 6 h under hypoxic conditions differs significantly from the control (p < 0,05)
Table 1. Differentially expressed genes involved in intracellular vesicular traffic
Note: * — the ratio of expression levels (arbitrary units) under hypoxic conditions to the expression levels under standard culture conditions. The data were gathered from microarrays. A negative value means that the expression of the studied gene decreased.
Table 2. Differentially expressed miRNAs regulating the expression of genes involved in intracellular vesicle transport
Note: * — the ratio of expression levels (arbitrary units) under hypoxic conditions to the expression levels under standard culture conditions; the data were generated by NGS. A negative value means that the expression of the studied miRNA decreased.