ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2021 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Experimental assessment of biological potential of collagen membranes in reconstruction of full-thickness hyaline cartilage defects
About authors
1 Pirogov Russian National Research Medical University, Moscow, Russia
2 OOO Aptos Group, Moscow, Russia
3 Doctor Vorontsov's Veterinary Center for Surgery and Oncology, Moscow, Russia
4 Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow, Russia
Correspondence should be addressed: Guram D. Lazishvili
Ostrovityanova, 1, 117997, Moscow; moc.liamg@zalmarug
About paper
Author contribution: Lazishvili GD — design of the experiment, participation in the experimental surgery, analysis of literature and experimental materials, article authoring; Yeghiazaryan KA — analysis of literature, experimental results; Nikishin DV — processing and analysis of the experimental data, article authoring; Vorontsov AA — execution of the experimental surgery; Klinov DV — Ortokeep collagen membrane design and development.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Center for Preclinical Research of Penza (Minutes № 1–19 of March 11, 2019). The animals were kept and used in compliance with the ethical standards and International requirements for humane treatment of laboratory (experimental) animals, as well as GOST R ISO 10993-1-2009 Medical Devices.
Received: 2021-07-19
Accepted: 2021-08-05
Published online: 2021-08-20
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Fig. 1. Stages of formation of full-thickness defects and implantation of collagen membranes. 1 — defect № 1 (implantation of the Ortokeep membrane); 2 — defect № 2 (implantation of the Chondro-Gide membrane); 3 — defect № 3 (no membrane implantation)
Fig. 2. Macro specimen at various timepoints post surgery. 1 — defect № 1 after implantation of the Ortokeep membrane; 2 — defect № 2 after implantation of the Chondro-Gide membrane; 3 — defect № 3, no membrane implantation
Fig. 3. Microscopic examination of defect № 3, control group; staining with hematoxylin and eosin, — 40 (A); Van Gieson's stain, ×40 (B). 1 — osteodystrophy; 2 — coarse fibrous connective tissue; 3 — developing "crater" defect in cartilage and bone tissues; 4 — groups of adipocytes filling the "crater" defect; 5 — bundles of collagen fibers between islets of adipocytes
Fig. 4. Microscopic examination of defect № 1 after implantation of the Ortokeep membrane; staining with hematoxylin and eosin, ×40 (A); Van Gieson's stain, ×40 (B). 1 — fibrin; 2 — coarse fibrous connective tissue; 3 — developing cylindrical defect, active neoosteogenesis evident at the edges; 4 — neochondrogenesis; 5 — the defect shrunk sharply with formation of a slit cavity in the center
Fig. 5. Microscopic examination of defect № 2 after implantation of the Chondro-Gide membrane, stained with hematoxylin and eosin, ×40 (A); Van Gieson's stain, ×40 (B). 1 — fibrin; 2 — coarse fibrous connective tissue; 3 — emerging bulbous defect, active neoosteogenesis evident along the edges; 4 — neochondrogenesis; 5 — the bed of the bone defect filled with adipocytes
Table. Dimensional characteristics of the histological structure of the defect's center, various types of treatment
