2021 / 06

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DOI: 10.24075/brsmu.2021.066

ORIGINAL RESEARCH

Significance of analysis of lipid extracts in cervical canal secretion for diagnosing of placenta-associated complications of pregnancy

Lomova NA, Chagovets VV, Tokareva AO, Dolgopolova EL, Karapetyan TE, Magomedova AP, Shmakov RG
About authors

Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

Correspondence should be addressed: Natalia A. Lomova
Akademika Oparina, 4, Moscow, 117997, Russia; ur.xednay@avomol-ahsatan

About paper

Funding: the study was supported through the state assignment of the Ministry of Health of the Russian Federation [State registration number AAAA-A18-118053190026-6].

Acknowledgements: the authors are grateful to Dr. V. E. Frankevich, Head of the Department of Systems Biology in Reproduction of the V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, for his help in organization of the study, systematic analysis and manuscript editing.

Author contribution: Lomova NA — analysis of clinical data, systematic analysis, manuscript authoring; Chagovets VV — metabolic analysis (mass spectrometry), statistical analysis of the data obtained, manuscript editing; Tokareva AO — metabolic analysis (mass spectrometry), mass spectrometry data processing; Dolgopolova EL — collection and preparation of biological fluids, statistical analysis of the results; Karapetyan TE — analysis of clinical data, statistical analysis of the results; Magomedova AP — collection and preparation of biological fluids; Shmakov RG — analysis of clinical data, systematic analysis, manuscript editing.

Compliance with ethical standards: the study was approved by the ethical committee of Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (Minutes #11 of November 11, 2021), conducted in accordance with the requirements of the Declaration of Helsinki, International Conference on Harmonization (ICH), Standards of Good Clinical Practice (GCP), Federal Law "On the Basics of Health Protection of Citizens in the Russian Federation"; all patients signed a voluntary informed consent to participate in the study.

Received: 2021-11-23 Accepted: 2021-12-20 Published online: 2021-12-29
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Fig. 1. Plotting of lipid levels. Significant differences between lipid levels, comparison of the normal and the IUGR groups, positive ion mode (A) and negative ion mode (B)
Fig. 2. Plotting of lipid levels. Significant differences between lipid levels, comparison of the normal and the PE groups, positive ion mode (A) and negative ion mode (B)
Fig. 3. Plotting of lipid levels. Significant differences between lipid levels, comparison of the PE and the IUGR groups, positive ion mode
Fig. 4. Operational curve plotted for the IUGR/Normal model based on the positive ion mode (A) and negative ion mode (B) data
Fig. 5. Operational curve plotted for the PE/Normal model (A), IUGR/PE model based on the positive ion mode data (B)
Table 1. Clinical characteristics of the groups
Table 2. Characteristics of the components used to build logistic regression models enabling allocation of the patients to the IUGR , PE and Normal (control) groups by the lipid profile established for the cervical canal secretion: independent variable, its coefficient β, confidence interval (CI) of β, Wald test, probability of coefficient value differing from P zero
Table 3. Characteristics of the logistic regression models enabling allocation of the patients to the IUGR, PE and Normal (control) groups by the lipid profile established for the cervical canal secretion. PPV — positive predictive value, NPV — negative predictive value