Novel Klebsiella pneumoniae virulent bacteriophage KPPK108.1 capable of infecting the K108 serotype strains

Evseev PV1, Shneider MM1, Mikhailova YuV2, Shelenkov AA2, Yanushevich YuG3, Karlova MG4, Moiseenko AV4, Sokolova OS4, Shagin DA3
About authors

1 Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia

2 Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow, Russia

3 Pirogov Russian National Research Medical University, Moscow, Russia

4 Lomonosov Moscow State University, Moscow, Russia

Correspondence should be addressed: Mikhail M. Shneider
Miklukho-Maklaya, 16/10, Moscow, 117997, Russia; ur.liam@nhs_mm

About paper

Funding: the study was funded by the Ministry of Health of the Russian Federation (EGISU R&D № 121052800048-3).

Acknowledgements: the authors wish to thank the Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Pirogov Russian National Research Medical University, for advice on research methods.

Author contribution: Shagin DA — research conceptualization, study management, manuscript writing; Evseev PV, Shelenkov AA — formal analysis of sequencing data, manuscript editing; Shneider MM — methodology, study management; Mikhailova YuV — sequencing, data validation; Yanushevich YuG, Moiseenko AV, Karlova MG — methodology; Sokolova OS — electron microscopy, methodology.

Received: 2021-12-08 Accepted: 2021-12-22 Published online: 2021-12-30

Multidrug-resistant Klebsiella pneumoniae strains are one of the major causes of nosocomial infections caused by the antibiotic-resistant bacteria. There are different options for dealing with this threat, among which is the clinical application of bacteriophages. The study was aimed to isolate and describe a virulent bactriophage, having the potential for therapeutic use. The standard phage biology and bioinformatic methods were used, which included the advanced techniques for protein structure prediction (AlphaFold software), and electron microscopy. The virulent podovirus KPPK108.1, being the member of genus Drulisvirus, which is able to specifically infect the K. pneumoniae strains with the KL108 type capsular polysaccharide, has been isolated from the wastewater. The sequence of the bactriophage genome has been defined, the biological properties have been investigated, and the genetic features have been described.

Keywords: bacteriophage, Klebsiella pneumoniae, capsular polysaccharide, depolymerase