ISSN Print 2500–1094
ISSN Online 2542–1204
Bulletin of RSMU
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Significance of analysis of lipid extracts in cervical canal secretion for diagnosing of placenta-associated complications of pregnancy
About authors
Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia
Correspondence should be addressed: Natalia A. Lomova
Akademika Oparina, 4, Moscow, 117997, Russia; ur.xednay@avomol-ahsatan
About paper
Funding: the study was supported through the state assignment of the Ministry of Health of the Russian Federation [State registration number AAAA-A18-118053190026-6].
Acknowledgements: the authors are grateful to Dr. V. E. Frankevich, Head of the Department of Systems Biology in Reproduction of the V. I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, for his help in organization of the study, systematic analysis and manuscript editing.
Author contribution: Lomova NA — analysis of clinical data, systematic analysis, manuscript authoring; Chagovets VV — metabolic analysis (mass spectrometry), statistical analysis of the data obtained, manuscript editing; Tokareva AO — metabolic analysis (mass spectrometry), mass spectrometry data processing; Dolgopolova EL — collection and preparation of biological fluids, statistical analysis of the results; Karapetyan TE — analysis of clinical data, statistical analysis of the results; Magomedova AP — collection and preparation of biological fluids; Shmakov RG — analysis of clinical data, systematic analysis, manuscript editing.
Compliance with ethical standards: the study was approved by the ethical committee of Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology (Minutes #11 of November 11, 2021), conducted in accordance with the requirements of the Declaration of Helsinki, International Conference on Harmonization (ICH), Standards of Good Clinical Practice (GCP), Federal Law "On the Basics of Health Protection of Citizens in the Russian Federation"; all patients signed a voluntary informed consent to participate in the study.
Received: 2021-11-23
Accepted: 2021-12-20
Published online: 2021-12-29
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Omics technologies hold great potential as the basis for development of the new diagnostic approaches in obstetrics. Cervicovaginal fluid (CVF) as part of the mother-placenta-fetus system can be used to diagnose obstetric complications. This study aimed to identify the features of lipid composition of the cervical canal secretion peculiar to Intrauterine Growth Restriction (IUGR) and preeclampsia (PE). We took CVF samples from 57 pregnant women and subjected them to an in-depth clinical-anamnestic and mass-spectrometric analysis. Lipid extracts of CVF were analyzed with a liquid chromatography system coupled with a mass analyzer. As a result, we identified 239 lipid compounds. In case of 17 lipids, mathematical analysis revealed significant differences between samples from women with normal pregnancy indicator values (normal group) and patients from the IUGR group (p < 0.05). As for the normal group and PE group patients, there were significant differences identified for 3 lipids (p < 0.05). Comparison of samples from the PE and IUGR groups yielded statistically significant differences in levels of two lipids (p < 0.05). Mainly, the lipids were oxylipins, sphingomyelins, triglycerides, and cardiolipins. The developed diagnostic model had the sensitivity of 0.81 and specificity of 0.91 (cut-off level — 0.50; AUC — 0.85). The data obtained are valuable in the context of development of the new methods of diagnosing placentaassociated complications of pregnancy and for understanding new mechanisms of pathogenesis of these complications.
Keywords: preeclampsia, prodnostic value, non-invasive diagnostics, fetal growth retardation, lipidomics