ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
METHOD
Sensors for analysis of drugs, drug-drug interactions, and catalytic activity of enzymes
About authors
1 Institute of Biomedical Chemistry (IBMC), Moscow, Russia
2 Pirogov Russian National Research Medical University, Moscow, Russia
Correspondence should be addressed: Lyubov E. Agafonova
Pogodinskaya, 10/8, Moscow, 119121, Russia; ur.liam@abulavonofaga
About paper
Funding: the study was carried out within the framework of the Russian Federation fundamental research program for the long-term period for 2021–2030.
Author contribution: Agafonova LE — experimental procedure, data processing, manuscript writing, building graphs; Bulko TV — sample preparation, experimental procedure; Kuzikov AV — statistical data processing, manuscript writing; Masamrekh RA — sample preparation, experimental procedure; Shumyantseva VV — concept, manuscript writing, data analysis.
Received: 2022-02-07
Accepted: 2022-02-21
Published online: 2022-02-28
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Fig. 1. Differential pulse voltammogram acquired on PGE in the 0.1 М potassium phosphate buffer containing 50 mМ NaCl, рН 7.4, in the abscence (---) or presence (-) of the 1 mМ drug А. Insert: relationship between the oxidation peak current and the drug А concentration
Fig. 2. А. Differential pulse voltammograms of drug I acquired on PGE in the 0.1 М potassium phosphate buffer containing 50 mМ NaCl, рН 7.4, in the concentration range of 0.5 mМ – 10 mМ. Insert: relationship between the oxidation peak current and the drug I concentration. B. Differential pulse voltammograms acquired PGE in the absence (---) or presence (-) of the 1 mМ drug I
Fig. 3. Differential pulse voltammograms of drug D acquired on PGE in the 0.1 М potassium phosphate buffer containing 50 mМ NaCl, рН 7.4, in the concentration range of 50 µM – 500 µM, with the drug D serum concentration of 100 µМ (-) (after protein precipitation, blood serum was diluted 10 times with the 0.1 М potassium phosphate buffer, 50 mМ NaCl, рН 7.4). Insert: relationship between the oxidation peak current and the drug D concentration
Fig. 4. Differential pulse voltammograms acquired on PGE in the 0.1 М potassium phosphate buffer containing 50 mМ NaCl, рН 7.4, in the absence (---) or presence of the 98 µМ drug М (-), 100 µМ drug D (-), 100 µМ drug D +
98 µМ drug М (-)
Fig. 5. Differential pulse voltammograms acquired on PGE in the 0.1 М potassium phosphate buffer containing 50 mМ NaCl, рН 7.4, in the presence of 25 µМ drug D (---), 200 µМ drug I (…), and the combination of 25 µМ drug D and 200 µМ drug I (-)
Table. Electroanalytical characteristics of drugs, obtained by differential pulse voltammetry on PGE in the 0.1 М potassium phosphate buffer containing 50 mМ NaCl, рН 7.4 (n = 4–5; p = 0.95)
