ORIGINAL RESEARCH
InlB protein secreted by Listeria monocytogenes controls the pathogen interaction with macrophages
1 Gamaleya National Research Center for Epidemiology and Microbiology, Moscow, Russia
2 Joint Institute for High Temperatures, Moscow, Russia
3 Pirogov Russian National Research Medical University, Moscow, Russia
4 Skolkovo Institute of Science and Technology, Moscow, Russia
Correspondence should be addressed: Yaroslava M. Chalenko
Gamaleya, 18, Moscow, 123098, Russia; ur.xednay@akazavalsoray
Funding: the study was supported by the Russian Science Foundation (project number 21-74-00105).
Author contribution: Chalenko YM — research planning, preparation and direct participation in all experiments, data interpretation and manuscript writing; Abdulkadieva MM, Safarova PV — macrophage infection assay; Kalinin EV — InlB expression analysis; Slonova DA — macrophage isolation and differentiation assay; Ermolaeva SA — research planning and manuscript writing.
Compliance with ethical standards: the research was conducted in compliance with the ethical principles of the World Medical Association Declaration of Helsinki.
- Murray E, Webb R, Swann M. A disease of rabbits characterized by a large mononuclear monocytosis, caused by a hitherto undescribed bacillus Bacterium monocytogenes. J Pathol Bacteriol. 1926; 29: 407–39.
- Vázquez-boland J, Kuhn M, Berche P, Chakraborty T, Domi G, González-zorn B, et al. Listeria Pathogenesis and Molecular Virulence Determinants Listeria Pathogenesis and Molecular Virulence Determinants. Clin Microbiol Rev. 2001; 14: 584–640.
- Allerberger F, Wagner M. Listeriosis: A resurgent foodborne infection. Clin Microbiol Infect. 2010; 16: 16–23.
- Braun L, Cossart P. Interactions between Listeria monocytogenes and host mammalian cells. Microbes Infect. 2000; 2: 803–11.
- Shen Y, Naujokas M, Park M, Ireton K. InIB-dependent internalization of Listeria is mediated by the Met receptor tyrosine kinase. Cell. 2000; 103: 501–10.
- Cossart P. Met, the HGF-SF receptor: another receptor for Listeria monocytogenes. Trends Microbiol. 2001; 9: 105–7.
- Organ S, Tsao M. An overview of the c-MET signaling pathway. Ther Adv Med Oncol. 2011; 3: 7–9.
- Chen Q, DeFrances MC, Zarnegar R. Induction of Met protooncogene (hepatocyte growth factor receptor) expression during human monocyte-macrophage differentiation. Cell Growth Differ. 1996; 7: 821–32.
- Nishikoba N, Kumagai K, Kanmura K, NakamuraY, Ono M, Eguchi H, et al. HGF-MET Signaling Shifts M1 Macrophages Toward an M2Like Phenotype Through PI3K-Mediated Induction of Arginase-1 Expression. Front Immunol. 2020; 11: 1–10.
- Ghebrehiwet B, Lim B, Peerschke E, Willis A, Reid K. Isolation, cDNA cloning, and overexpression of a 33-kDa cell surface glycoprotein that binds to the globular heads of C1q. J Exp Med. 1994; 179: 1809–21.
- Ghebrehiwet B, Peerschke E. Structure and function of gC1q-R a multiligand binding membrane protein. Immunobiology. 1998; 199: 225–38.
- Chalenko Y, Kalinin E, Marchenkov V, Sysolyatina E, Surin A, Sobyanin K, et al. Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R. Int J Mol Sci. 2019; 20: 1–15.
- ChalenkoY, Kolbasova O, Pivova E, Abdulkadieva M, Povolyaeva O, Kalinin E. Listeria monocytogenes Invasion Into Sheep Kidney Epithelial Cells Depends on InlB, and Invasion Efficiency Is Modulated by Phylogenetically Defined InlB Isoforms. Front Microbiol. 2022; 13: 1–14.
- Sobyanin K, Sysolyatina E, Krivozubov M, Chalenko Y, Karyagina A, Ermolaeva S. Naturally occurring InlB variants that support intragastric Listeria monocytogenes infection in mice. FEMS Microbiol Lett. 2017: 1; 1–8.
- Nepomuceno R, Tenner A. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998; 160: 1929–35.
- Mansell A, Braun L, Cossart P, O'Neill L. A novel function of InIB from Listeria monocytogenes: activation of NF-kappaB in J774 macrophages. Cell Microbiol. 2000; 2: 127–36.
- Calame D, Mueller-Ortiz S, Wetsel R. Innate and adaptive immunologic functions of complement in the host response to Listeria monocytogenes infection. Immunobiology. 2016; 221: 1407–17.
- Fuss I, Kanof M, Smith P, Zola H. Isolation of whole mononuclear cells from peripheral blood and cord blood. Curr Protoc Immunol. 2009; 85: 1.1–7.1.8.
- Huang X, Li Y, Fu M, Xin H. Polarizing Macrophages In Vitro. Germain Rousselet (ed.), Macrophages: Methods and Protocols. Methods in Molecular Biology. 2018; 1784: 119–26.
- Werbrouck H, Grijspeerdt K, Botteldoorn N, Pamel E, Rijpens N, Damme J, et al. Differential inlA and inlB Expression and Interaction with Human Intestinal and Liver Cells by Listeria monocytogenes Strains of Different Origins. Appl Environ Microbiol. 2006; 72 (6): 3862–71.