ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
Copyright: © 2023 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).
ORIGINAL RESEARCH
Activation of microglia in the brain of spontaneously hypertensive rats
About authors
1 Institute of Experimental Medicine, St Petersburg, Russia
2 St Petersburg State University, St Petersburg, Russia
Correspondence should be addressed: Valeria V. Guselnikova
Acad. Pavlov, 12, Saint-Petersburg, 197376, Russia; ur.xednay@aiirelav.avocinlesug
About paper
Funding: the study was funded by the Russian Science Foundation, project № 22-25-00105, https://rscf.ru/project/22-25-00105/.
Author contribution: Guselnikova VV — literature analysis, analysis and interpretation of the results, preparation of the manuscript; Razenkova VA — development of protocols for immunofluorescent reactions, confocal laser microscopy; Sufieva DA — histological examination of biological material, performing immunohistochemical reactions for light microscopy; Korzhevskii DE — concept development, research planning, manuscript editing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Federal State Budgetary Scientific Institution "IEM" (protocol № 1/22 dated February 18, 2022, protocol № 3/19 dated April 25, 2019), and was conducted in accordance with the provisions of the Declaration of Helsinki (2013)
Received: 2023-06-05
Accepted: 2023-06-20
Published online: 2023-06-27
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Fig. 1. Microglia in the rat brain. WKY — Wistar–Kyoto normotensive rat line; SHR — spontaneously hypertensive rat line; Cortex — area of the large cerebral cortex;
Striatum — striatum (corpus striatum); WM — subcortical white matter; SFO — subfornical organ. Immunohistochemical reaction for Iba1, counterstained with alum
hematoxylin. Scale bar is 50 µm
Fig. 2. Subfornical organ of a spontaneously hypertensive rat. Double immunofluorescence reaction for Iba1 (green fluorescence) and CD68 (red fluorescence). The yellow color marks the colocalization areas of the green and red channels. Figure B is an enlarged fragment of Figure A (white box). SFO, subfornical organ; WM, white matter; asterisk, blood vessel; arrowhead indicates CD68-rich cell. Confocal microscopy. А. Panoramic image; the scale bar is 100 µm. B. Three-dimensional reconstruction of a series of optical slices, grid cell size 10 × 10 µm
