2023 / 03

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DOI: 10.24075/brsmu.2023.024

ORIGINAL RESEARCH

Activation of microglia in the brain of spontaneously hypertensive rats

Guselnikova VV1,2, Razenkova VA1, Sufieva DA1, Korzhevskii DE1
About authors

1 Institute of Experimental Medicine, St Petersburg, Russia

2 St Petersburg State University, St Petersburg, Russia

Correspondence should be addressed: Valeria V. Guselnikova
Acad. Pavlov, 12, Saint-Petersburg, 197376, Russia; ur.xednay@aiirelav.avocinlesug

About paper

Funding: the study was funded by the Russian Science Foundation, project № 22-25-00105, https://rscf.ru/project/22-25-00105/.

Author contribution: Guselnikova VV — literature analysis, analysis and interpretation of the results, preparation of the manuscript; Razenkova VA — development of protocols for immunofluorescent reactions, confocal laser microscopy; Sufieva DA — histological examination of biological material, performing immunohistochemical reactions for light microscopy; Korzhevskii DE — concept development, research planning, manuscript editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Federal State Budgetary Scientific Institution "IEM" (protocol № 1/22 dated February 18, 2022, protocol № 3/19 dated April 25, 2019), and was conducted in accordance with the provisions of the Declaration of Helsinki (2013)

Received: 2023-06-05 Accepted: 2023-06-20 Published online: 2023-06-27
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Fig. 1. Microglia in the rat brain. WKY — Wistar–Kyoto normotensive rat line; SHR — spontaneously hypertensive rat line; Cortex — area of the large cerebral cortex; Striatum — striatum (corpus striatum); WM — subcortical white matter; SFO — subfornical organ. Immunohistochemical reaction for Iba1, counterstained with alum hematoxylin. Scale bar is 50 µm
Fig. 2. Subfornical organ of a spontaneously hypertensive rat. Double immunofluorescence reaction for Iba1 (green fluorescence) and CD68 (red fluorescence). The yellow color marks the colocalization areas of the green and red channels. Figure B is an enlarged fragment of Figure A (white box). SFO, subfornical organ; WM, white matter; asterisk, blood vessel; arrowhead indicates CD68-rich cell. Confocal microscopy. А. Panoramic image; the scale bar is 100 µm. B. Three-dimensional reconstruction of a series of optical slices, grid cell size 10 × 10 µm