ISSN Print 2500–1094
ISSN Online 2542–1204
BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)
ORIGINAL RESEARCH
Identification of microglia and macrophages using antibodies to various sequences of the Iba-1 protein
About authors
Institute of Experimental Medicine, St Petersburg, Russia
Correspondence should be addressed: Valeria A. Razenkova
Akademika Pavlova, 12, Saint Petersburg, 197376, Russia; ur.xednay@zar.ayirelav
About paper
Funding: the study received financial support from the Russian Science Foundation, project No. 24-15-00032, https://rscf.ru/en/project/24-15-00032/
Author contribution: Razenkova VA — literature analysis, interpretation of the results, manuscript authoring; Kirik OV — analysis of the results, text editing, preparation of figures; Pavlova VS — histology of biological material, immunohistochemical reactions setup; Korzhevskii DE — study conceptualization, planning, text editing.
Compliance with ethical standards: the study was approved by the Ethics Committee of the Institute of Experimental Medicine (Protocol № 2/24 of April 25, 2024), and conducted in full compliance with the provisions of the Declaration of Helsinki (2013).
Received: 2024-05-17
Accepted: 2024-06-05
Published online: 2024-06-29
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Fig. 1. Iba-1 in rat cells, identified with polyclonal goat (A, C, E), monoclonal rabbit (B, D, E) antibodies. A–D. Brain preparations (arrows indicate microglia). E–F. Convoluted seminiferous tubules. Arrows point to Iba-1-containing cells in the interstitium, double arrows indicate such in the germinal epithelium, asterisk — in the tubule's lumen. Scale: 50 microns (A, B, E, F), 20 microns (C, D)
