Copyright: © 2024 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

Identification of microglia and macrophages using antibodies to various sequences of the Iba-1 protein

Razenkova VA, Kirik OV, Pavlova VS, Korzhevskii DE
About authors

Institute of Experimental Medicine, St Petersburg, Russia

Correspondence should be addressed: Valeria A. Razenkova
Akademika Pavlova, 12, Saint Petersburg, 197376, Russia; ur.xednay@zar.ayirelav

About paper

Funding: the study received financial support from the Russian Science Foundation, project No. 24-15-00032, https://rscf.ru/en/project/24-15-00032/

Author contribution: Razenkova VA — literature analysis, interpretation of the results, manuscript authoring; Kirik OV — analysis of the results, text editing, preparation of figures; Pavlova VS — histology of biological material, immunohistochemical reactions setup; Korzhevskii DE — study conceptualization, planning, text editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Institute of Experimental Medicine (Protocol № 2/24 of April 25, 2024), and conducted in full compliance with the provisions of the Declaration of Helsinki (2013).

Received: 2024-05-17 Accepted: 2024-06-05 Published online: 2024-06-29
|
Fig. 1. Iba-1 in rat cells, identified with polyclonal goat (A, C, E), monoclonal rabbit (B, D, E) antibodies. A–D. Brain preparations (arrows indicate microglia). E–F. Convoluted seminiferous tubules. Arrows point to Iba-1-containing cells in the interstitium, double arrows indicate such in the germinal epithelium, asterisk — in the tubule's lumen. Scale: 50 microns (A, B, E, F), 20 microns (C, D)