2024 / 03

DOI: 10.24075/brsmu.2024.026

ORIGINAL RESEARCH

Identification of microglia and macrophages using antibodies to various sequences of the Iba-1 protein

Razenkova VA, Kirik OV, Pavlova VS, Korzhevskii DE
About authors

Institute of Experimental Medicine, St Petersburg, Russia

Correspondence should be addressed: Valeria A. Razenkova
Akademika Pavlova, 12, Saint Petersburg, 197376, Russia; ur.xednay@zar.ayirelav

About paper

Funding: the study received financial support from the Russian Science Foundation, project No. 24-15-00032, https://rscf.ru/project/24-15-00032/

Author contribution: Razenkova VA — literature analysis, interpretation of the results, manuscript authoring; Kirik OV — analysis of the results, text editing, preparation of figures; Pavlova VS — histology of biological material, immunohistochemical reactions setup; Korzhevskii DE — study conceptualization, planning, text editing.

Compliance with ethical standards: the study was approved by the Ethics Committee of the Institute of Experimental Medicine (Protocol № 2/24 of April 25, 2024), and conducted in full compliance with the provisions of the Declaration of Helsinki (2013).

Received: 2024-05-17 Accepted: 2024-06-05 Published online: 2024-06-29
|
  1. Fields RD, Araque A, Johansen-Berg H, Lim SS, Lynch G, Nave KA, et al. Glial biology in learning and cognition. Neuroscientist. 2014; 20 (5): 426–31. DOI: 10.1177/1073858413504465.
  2. Li Q, Barres BA. Microglia and macrophages in brain homeostasis and disease. Nat Rev Immunol. 2018; 18 (4): 225–242. DOI: 10.1038/nri.2017.125.
  3. Kreutzberg GW. Microglia: a sensor for pathological events in the CNS. Trends Neurosci. 1996; 19 (8): 312–8. DOI: 10.1016/0166-2236(96)10049-7.
  4. Jin X, Yamashita T. Microglia in central nervous system repair after injury. J Biochem. 2016; 159 (5): 491–96. DOI: 10.1093/JB/MVW009.
  5. Guselnikova VV, Razenkova VA, Sufieva DA, Korzhevskii DE. Activation of microglia in the brain of spontaneously hypertensive rats. Bulletin of RSMU. 2023; (3): 49–55. DOI: 10.24075/brsmu.2023.024.
  6. Imai Y, Ibata I, Ito D, Ohsawa K, Kohsaka S. A novel gene iba1 in the major histocompatibility complex class III region encoding an EF hand protein expressed in a monocytic lineage. Biochem Biophys Res Commun. 1996; 224 (3): 855–62. DOI: 10.1006/BBRC.1996.1112.
  7. Utans U, Arceci RJ, Yamashita Y, Russell ME. Cloning and characterization of allograft inflammatory factor-1: a novel macrophage factor identified in rat cardiac allografts with chronic rejection. J Clin Invest. 1995; 95 (6): 2954–62. DOI: 10.1172/JCI118003.
  8. Lituma PJ, Woo E, O’Hara BF, Castillo PE, Sibinga NES, Nandi S. Altered synaptic connectivity and brain function in mice lacking microglial adapter protein Iba1. Proc Natl Acad Sci U S A. 2021; 118 (46): e2115539118 [Internet] 2021 Nov 16 [cited 2024 May 13]. Available from: https://www.pnas.org/doi/10.1073/pnas.2115539118?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref. org&rfr_dat=cr_pub%20%200pubmed.
  9. Deininger MH, Meyermann R, Schluesener HJ. The allograft inflammatory factor-1 family of proteins. FEBS Lett. 2002; 514 (2–3): 115–21. DOI:10.1016/S0014-5793(02)02430-4
  10. Vizioli J, Verri T, Pagliara P. Allograft Inflammatory Factor-1 in Metazoans: Focus on Invertebrates. Biology (Basel). 2020; 9 (11): 1–14. DOI: 10.3390/BIOLOGY9110355.
  11. Del Galdo F, Maul GG, Jiménez SA, Artlett CM. Expression of allograft inflammatory factor 1 in tissues from patients with systemic sclerosis and in vitro differential expression of its isoforms in response to transforming growth factor beta. Arthritis Rheum. 2006; 54 (8): 2616–25. DOI: 10.1002/ART.22010.
  12. Slim FA, Ouellette G, Ennour-Idrissi K, Jacob S, Diorio C, Durocher F. An isoform of AIF1 involved in breast cancer. Cancer Cell Int. 2018; 18 (1): 167 [Internet] 2018 Oct 22 [cited 2024 May 13]. Available from: https://cancerci.biomedcentral.com/articles/10.1186/s12935-018-0663-3.
  13. Broglio L, Erne B, Tolnay M, Schaeren-Wiemers N, Fuhr P, Steck AJ, et al. Allograft inflammatory factor-1: a pathogenetic factor for vasculitic neuropathy. Muscle Nerve. 2008; 38 (4): 1272–9. DOI: 10.1002/MUS.21033.
  14. Liu S, Tan WY, Chen QR, Chen XP, Fu K, Zhao YY, et al. Daintain/AIF-1 promotes breast cancer proliferation via activation of the NFkappaB/cyclin D1 pathway and facilitates tumor growth. Cancer Sci. 2008; 99 (5): 952–7. DOI: 10.1111/J.1349-7006.2008.00787.X.
  15. Jia S, Du Z, Jiang H, Huang X, Chen Z, Chen N. Daintain/AIF-1 accelerates the activation of insulin-like growth factor-1 receptor signaling pathway in HepG2 cells. Oncol Rep. 2015; 34 (1): 511– 7. DOI: 10.3892/OR.2015.4002.
  16. Köhler C. Allograft inflammatory factor-1/Ionized calciumbinding adapter molecule 1 is specifically expressed by most subpopulations of macrophages and spermatids in testis. Cell Tissue Res. 2007; 330 (2): 291–302. DOI: 10.1007/S00441-007-0474-7.
  17. Zhang S, Gong P, Zhang J, Mao X, Zhao Y, Wang H, et al. Specific frequency electroacupuncture stimulation transiently enhances the permeability of the blood-brain barrier and induces tight junction changes. Front Neurosci. 2020; 14: 582324 [Internet] 2020 Oct 6 [cited 2024 May 13]. Available from: https://www.frontiersin.org/journals/neuroscience/articles/10.3389/fnins.2020.582324/full.
  18. Nikitina IA, Razenkova VA, Kirik OV, Korzhevskij DJe. Vyjavlenie populjacii kletok Kupfera v pecheni krysy s ispol'zovaniem monoklonal'nyh i poliklonal'nyh antitel k mikroglial'nomu markeru Iba-1. Medicinskij akademicheskij zhurnal. 2023; 23 (1): 85–94. DOI: 10.17816/MAJ133649. Russian.
  19. Liu C, Zhao X-M, Wang Q, Du TT, Zhang MX, Wang HZ, et al. Astrocyte-derived SerpinA3N promotes neuroinflammation and epileptic seizures by activating the NF-κB signaling pathway in mice with temporal lobe epilepsy. J Neuroinflammation. 2023; 20 (1): 161 [Internet] 2023 Jul 8 [cited 2024 May 13]. Available from: https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-023-02840-8.
  20. Buchwalow I, Samoilova V, Boecker W, Tiemann M. Non-specific binding of antibodies in immunohistochemistry: fallacies and facts. Sci Rep. 2011; 1: 28 [Internet] 2011 Jul 1 [cited 2024 May 13]. Available from: https://www.nature.com/articles/srep00028.
  21. Ohsawa K, Imai Y, Sasaki Y, Kohsaka S. Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity. J Neurochem. 2004; 88 (4): 844–56. DOI: 10.1046/J.1471-4159.2003.02213.X.
  22. Gheorghe RO, Grosu AV, Magercu M, Ghenghea MS, Zbarcea CE, Tanase A, et al. Switching rat resident macrophages from M1 to M2 phenotype by Iba1 silencing has analgesic effects in SNL-induced neuropathic pain. Int J Mol Sci. 2023; 24 (21): 15831 [Internet] 2023 Oct 31 [cited 2024 May 13]. Available from: https://www.mdpi.com/1422-0067/24/21/15831.
  23. Yoshii D, Nakagawa T, Komohara Y, Kawaguchi H, Yamada S, Tanimoto A. Phenotypic changes in macrophage activation in a model of nonalcoholic fatty liver disease using microminipigs. J Atheroscler Thromb. 2021; 28 (8): 844–51. DOI: 10.5551/JAT.57703.
  24. Donovan KM, Leidinger MR, McQuillen LP, Goeken JA, Hogan CM, Harwani SC, et al. Allograft inflammatory factor 1 as an immunohistochemical marker for macrophages in multiple tissues and laboratory animal species. Comp Med. 2018; 68 (5): 341–48. DOI: 10.30802/AALAS-CM-18-000017.
  25. Hu X, Buhl CS, Sjogaard MB, Schousboe K, Mizrak HI, Kufaishi H, et al. Structural changes of cutaneous immune cells in patients with type 1 diabetes and their relationship with diabetic polyneuropathy. Neurol Neuroimmunol neuroinflammation. 2023; 10 (5): e200144 [Internet] 2023 Aug 1 [cited 2024 May 13]. Available from: https://www.neurology.org/doi/10.1212/NXI.0000000000200144?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed.
  26. Son NV, Chambers JK, Dung LT, Kishimoto TE, Nishimura M, Kita C, Takada Y, et al. Histological and immunohistochemical features of normal histiocytes and Langerhans cells, and histiocytic sarcomas in four-toed hedgehogs (Atelerix albiventris). J Comp Pathol. 2020; 178: 32–40. DOI: 10.1016/J.JCPA.2020.06.009.
  27. Özbek M, Beyaz F, Ergün E, Ergün L, Karaca H, Cabir A, et al. Identification of some calcium binding proteins and neural cell markers in rat testis and epididymis during postnatal development. Andrologia. 2022; 54 (11): e14633 [Internet] 2022 Nov 14 [cited 2024 May 13]. Available from: https://onlinelibrary.wiley.com/doi/10.1111/and.14633.
  28. Iida H, Doiguchi M, Yamashita H, et al. Spermatid-specific expression of Iba1, an ionized calcium binding adapter molecule-1, in rat testis. Biol Reprod. 2001; 64 (4): 1138–46. DOI: 10.1095/BIOLREPROD64.4.1138.
  29. Saleh H, Nassar AMK, Noreldin AE, Samak D, Elshony N, Wasef L, et al. Chemo-protective potential of cerium oxide nanoparticles against Fipronil-induced oxidative stress, apoptosis, inflammation and reproductive dysfunction in male white albino rats. 2020; 25 (15): 3479 [Internet] 2020 Jul 31 [cited 2024 May 13]. Available from: https://www.mdpi.com/1420-3049/25/15/3479.