2026 / 02

DOI: 10.24075/brsmu.2026.011
Copyright: © 2026 by the authors. Licensee: Pirogov University.
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (CC BY).

ORIGINAL RESEARCH

A reliable and reproducible multiplex RT-qPCR assay for mTOR gene expression analysis

Kornilov DO1 , Simarzina VM1 , Bekhter AA1 , Maslakov GP2 , Nechaeva DM1 , Karyakina AE1 , Fadeev FA1,3 , Voroshilina ES1,4 , Zornikov DL1
About authors

1 Ural State Medical University, Yekaterinburg, Russia

2 Institute of Cytology of the Russian Academy of Sciences, Saint Petersburg, Russia

3 State Autonomous Health Institution of the Sverdlovsk Region “Center for Specialized Types of Medical Care ‘Institute of Medical Cellular Technologies’”, Yekaterinburg, Russia

4 Medical Center “Garmoniya”, Yekaterinburg, Russia

Correspondence should be addressed: Daniil O. Kornilov
Klyuchevskaya, 17, Yekaterinburg, 620109, Russia; moc.liamg@linrokvolinad

About paper

Acknowledgments: The authors express their gratitude to the staff of the Laboratory of Enteral Viral Infections, Federal Budgetary Institution of Science “Federal Scientific Research Institute of Viral Infections ‘Virome’” of Rospotrebnadzor, for their assistance in organizing the work.

Author contribution: Kornilov DO — conceptualization, methodology, writing — original draft; Simarzina VM — investigation, methodology, visualization; Bekhter AA — investigation, visualization; Maslakov GP — investigation, software, validation; Nechaeva DM — methodology, writing — review and editing; Kariakina AE — methodology, writing — review and editing; Fadeev FA — resources, writing — review and editing; Voroshilina ES — supervision, project administration; Zornikov DL — supervision, visualization, formal analysis, writing — review and editing.

Compliance with ethical standards: The study was approved by the Local Ethical Committee of the Ural State Medical University, Yekaterinburg, Russia (Protocol No. 6 dated October 18, 2024). Informed consent was not applicable, as commercial cell lines were used.

Received: 2026-03-11 Accepted: 2026-03-25 Published online: 2026-03-25
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