In recent years, the number of emergencies, armed conflicts, and household injuries has increased, which has elevated the risk of thermal burns for a broad segment of the population. Design and testing of new, safe and efficient burn-healing drugs remains a relevant task. This study aimed to evaluate the burn — healing effects of a new cream containing polyprenols isolated from Ginkgo biloba L. in a rat thermal-burn model. The cream's active ingredient is a highly purified (≥ 95%) Ginkgo biloba L. polyprenols, and the excipients are lipids (phosphatidylcholine, phosphatidylserine) with tocopherol antioxidants. The experimental animals were 32 male Wistar rats weighing 280–320 g. We evaluated the effect of the new polyprenol cream and the reference preparations methyluracil and dexpanthenol on the dynamics of the skin lesion area in rats with grade III-B skin burns. The experiment showed that, on day 21 after the burn, the new cream reduced the wound area in rats by 2.3 times compared with the controls. Morphological examination showed that the new cream had a 1.3-fold better burn-healing effect than the comparison drug methyluracil (dioxomethyltetrahydropyrimidine). Further preclinical study of the cream as a potential burn and wound healing agent seems promising.