Bulletin of RSMU

ISSN Print 2500–1094 ISSN Online 2542–1204

BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)

Titov KS, Demidov LV, Shubina Izh, Khaylenko VA, Kiselevskiy MV, Vikhrova AS

The study evaluated clinical efficacy and safety of the use of cells antitumor immunotherapy methods — vaccines based on autologic dendritic and allogenic LAK-cells in combination with recombinant interleukin-2 (IL-2). The clinical study involved cancer patients with skin melanoma of grades III-IV and patients with metastatic effusions resistant to systemic chemotherapy. The data led to the conclusion of safety, immune competence and reasonable effectiveness of DC-based vaccines in therapeutic and prophylactic schemes in patients with skin melanoma. The results showed that intra-cavity IL-2/LAK-immunotherapy was highly effective and well tolerated in treatment of patients with metastatic effusions.

Received: 2014-02-06

Published online: 2017-01-05

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Shirikorad VI, Peterson SB, Chulkova SV, Volodin DI, Kapustin VV, Egorova AV, Lepkova NN, Benevsky AI

The paper is devoted to a review and one of the actual topics of oncology — diagnosis of locally advanced prostate cancer. It provides the current status of the problem and highlights the major debated parties of the issue. The article describes the main methods of diagnosis: digital rectal examination, prostate specific antigen study, ultrasound, magnetic resonance imaging, biopsy of the prostate.

Received: 2013-09-18

Published online: 2017-01-05

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Runikhin AYu, Poryadin GV, Savchuk VI

The aim of the study was the evaluation of prostaglandin E2 (PGE2) influence on cholinergic cardiac innervation. Our experimental investigation established that PGE2 induced cardiodepressor effect in normotensive cats (n = 88) due to vagus nerves activation. PGE2 stimulated vagal C-afferent fibers thus leading to vagotonia. Vagotonic effect of PGE2 is one of the mechanisms for myocardium contractility and antihypertensive action. PGE2 modulating cholinergic influence may improve blood pressure control and morphology and function of vital organs.

Received: 2013-09-02

Published online: 2017-01-05

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Zaiko MV, Teselkin YuO, Pavlova LA, Kozin SV

In the study the ability of dihydroquercetin to inhibit the development of free radical reactions in the process of storing a new animal extracted product «Splenaktiv» (SA), which is a freeze-dried aqueous extract of the cattle spleen, was investigated. SA was prepared without (SA-1) and with dihydroquercetin supplement as an antioxidant (SA-2). The antioxidant activity of SA-1 and SA-2 and the content of lipid peroxidation product — malondialdehyde — were determined before and after storage for 2 years. It was found that after the period of storage of the selected antioxidant activity of the animal extracted product SA-1 decreased by10 times, and the content of malondialdehyde increased by 2 times as compared to baseline (p <0.01), whereas in the case of SA-2 the appropriate parameters practically did not change. Thus, the use of dihydroquercetin as an antioxidant in the composition of SA inhibits the development of oxidation processes and increases its shelf-life.

Received: 2014-01-27

Published online: 2017-01-05

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Chekhani NR, Pavlova LA, Kozin SV, Teselkin YuO

The influence of natural polyphenolic compounds included in a new collection of medicinal plant products on the physical endurance of mice in the re-swim test was studied. It was found out that the course application of aqueous extract from the plant collection in the experimental group of mice did not effect the duration of the primary swim, but increased to 1,7 times the length of re-swimming against the same parameter in the control group (p <0.001). This result demonstrates the positive effect of the proposed plant collection on the recover processes after physical activity, i.e. its actoprotective action. It is assumed that the actoprotective action of the medicinal plant collection is due to antioxidant properties of biologically active substances in its composition.

Received: 2013-12-25

Published online: 2017-01-05

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Guglya EB

Basic principles of liquid chromatography-tandem mass spectrometry method (LC-MS/MS) and its role in drug discovery and preclinical studies are reviewed. Three stages and components of the method in modern bioanalysis application are considered — sample preparation, liquid chromatography and mass spectrometry. The method trends and features for early drug screening and subsequent preclinical study of pharmacokinetics of drugs are discussed.

Received: 2014-01-22

Published online: 2017-01-05

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Gimranova GG, Bakirov AB, Karimova LK, Beygul NA, Shaykhlislamova ER

We have assessed occupational risks among oil extraction workers using hygienic and medico-biological criteria. In modern oil extraction enterprises, a complex of work environment and work process factors includes occupational noise, vibration, hazardous chemicals impact, outdoor air negative indicators, work severity and intensity. Working conditions in certain occupations are characterized by a combination of predominating factors. Their intensity is of class 3 (1-4 degree) hazards. The presence of hazardous factors leads to the development of occupational and work-related diseases among workers. The highest health occupational risk level is detected in drillers, assistant drillers, operators of major, underground drill hole repairs, and machine operators. Optimization measures for occupational risk management have been developed.

Received: 2013-11-11

Published online: 2017-01-05

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Badamshina GG, Karimova LK, Timasheva GV, Bakirov AB

A complex clinical-hygienic study in employees of a petrochemical complex for the production of synthetic resins was conducted. The main hazardous factor in the working environment under the study was a chemical production factor which was represented by 2–3 class hazards. In general, the working conditions were closely matched to class 3.3 according to the level of a chemical factor. The priority classes of chronic non-infectious diseases among petrochemical workers revealed statistical evidence for association of the disease with the work, characterizing the professional risk to the health of the workers.

Received: 2013-11-11

Published online: 2017-01-05

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