Bulletin of RSMU

ISSN Print 2500–1094 ISSN Online 2542–1204

BIOMEDICAL JOURNAL OF PIROGOV UNIVERSITY (MOSCOW, RUSSIA)

Kruglikov GG, Suslov VB, Likhacheva LM, Stranzha NB, Oettinger AP

Chronic inflammatory reactions in the lungs lead to circulatory problems in the microvessels, causing increased permeability of endothelial cells, edema and interstitial tissue hypoxia and respiratory tracts of lungs. Inflammation is kept by constant secretion of cytokines from specific granules of mast cells and eosinophils. Protective immune response is implemented by blast transformation of lymphocytes into plasma cells secreting antibodies. With pneumoconioses incomplete phagocytosis of foreign particles, permanent destruction of macrophages and tissue saturation with decay products stimulate an autoimmune response and the intensity of fibrosis.

Received: 2012-06-27

Published online: 2017-01-05

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Azova MM, Blagonravov ML, Frolov VA, Gigani OB, Gigani OO

The rates of cardiomyocyte apoptosis and myocardial hypertrophy were investigated in spontaneously hypertensive rats by TUNEL-assay and evaluation of nuclear-cytoplasmic ratio relatively. It was found that genetic hypertension in rats was accompanied by a significant increase in apoptotic index of ventricular cardiomyocytes, and the dynamics of programmed cell death in the right and left ventricles was different. Myocardial hypertrophy began to develop in SHR rats within the first weeks of life and was associated with reduced apoptosis rate. Moreover its effect on the programmed death of cardiomyocytes was more marked in the left ventricle of the heart.

Received: 2012-06-25

Published online: 2017-01-05

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Chekhani NR, Teselkin YuO, Pavlova LA, Kozin SV, Lyubitsky OB

In this study the antioxidant activity (AOA) of water extracts of some plants used in Tuva ethnomedicine was investigated. As a model system there was used a system in which the reaction of free radical oxidation of luminol was induced by adding 2,2'-azobis(2-amidinopropane) dihydrochloride (ABAP). The process of luminol oxidation was observed by recording its chemiluminescence. It was found that addition of water extracts from the studied plants to АВАР-luminol system resulted in the inhibition of luminol chemiluminescence and the appearance of the latent period, the duration of which was directly proportional to the amount of the added sample. Water extracts of herb meadowsweet (Filipendula ulmaria L.) had the highest AOA, and the least — the leaves of warty birch (Betula pendula Roth.) and black currant (Ribes nigrum L.). It is assumed that the AOA of water plants extracts is due to phenol and polyphenol compounds.

Received: 2012-07-09

Published online: 2017-01-05

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Nikolin AA, Krupina SI, Arkhipov DE, Kramarova EP, Korlyukov AA, Shkoporov AN, Shipov AG, Kafarskaya LI, Baukov YuI, Negrebetsky VV

Eight novel hypercoordinated silicon compounds were synthesized. Their structures were confirmed by multinuclear spectroscopy of nuclear magnetic resonance, infrared spectroscopy and X-ray diffraction study. The prognosis of biological activity indicated that complexes of hypercoordinated silicon could be screened as cardioprotective agents, and drugs for side (lateral) amiotrophic sclerosis treatment based on these complexes could be developed. All studied compounds had low predicted toxicity, which was confirmed by cytotoxicity tests on the model of tissue and bacterial cultures.

Received: 2012-07-11

Published online: 2017-01-05

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Ioutsi VA, Sokolov SA, Semivrazhskaya OO, Apenova MG, Belov NM, Kovalev SV, Shevchenko VE, Negrebetsky VV

Synthesis and investigation of the antioxidative properties of four new fullerene C60 derivatives are reported. Two new synthetic procedures of the fulleropyrrolidine derivatives preparation were developed. All structures were confirmed by means of nuclear magnetic resonance spectroscopy and infrared spectroscopy, mass-spectrometry with laser desorption/ionization and high-resolution mass-spectrometry. Antioxidative properties were investigated by determination of peroxide number using the standard procedure.

Received: 2012-05-23

Published online: 2017-01-05

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Usanova EA, Chausova SV, Filatov OYu, Krasyuk OV, Efimov BA, Balyakin YuV

In this article there was estimated a priming effect of Staphylococcus aureus and Klebsiella pneumoniae at the polymorphonuclear leukocytes by measuring luminal- and lucigenin amplified chemiluminescence of donors' blood. It was shown that bacterial antigens of both microorganisms stimulated oxidant functions of leukocytes.

Received: 2011-12-06

Published online: 2017-01-05

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Shatalov PA, Sukhorukov VS, Kharlamov DA, Brydun AV, Glinkina VV, Vlodavets DV, Vinogradskaya IS, Suslov VB, Likhacheva LM

Various methods of muscle biopsy morphological analysis are very important in the differential diagnostics of congenital myopathies. Not all neuromuscular diseases are identified by clear diagnostic criteria of morphological changes. The aim of this study was to search for electron microscopic markers, effective for the differential diagnosis of central core myopathy. Muscle biopsies were investigated in 56 patients aged from 1 to 21 years with diagnosis of central core myopathy (24 patients), multicore disease (6 patients), sarcotubular myopathy (10 patients), mitochondrial myopathy (16 patients). There were used methods of light (including histochemical) and electron microscopy. There were revealed basic electron-microscopic characteristics that could be used for differential diagnosis of congenital myopathies: central core, multicore and sarcotubular myopathies, as well as their morphological differences from mitochondrial myopathies.

Received: 2012-06-15

Published online: 2017-01-05

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Dubrovin IP, Komissarova SV, Turygina SA

Received: 2011-12-09

Published online: 2017-01-05

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