ORIGINAL RESEARCH

Genetic polymorphism of Staphylococcus epidermidis strains in patients of the Neonatal Intensive Care Unit

About authors

1 Department of Microbiology and Clinical Pharmacology,
Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

2 Department of Clinical and Molecular Genetics,
Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

3 Department of Neonatology and Pediatrics,
Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia

Correspondence should be addressed: Alexey B. Gordeev
ul. Akademika Oparina, d. 4, Moscow, Russia, 117997; ur.sertorp.agev@weedrog

About paper

Funding: this study was supported by the Grant Agreement No. 14.607.21.0019 of the Ministry of Science and Education of the Russian Federation
dated June 5, 2014 (ID 2014-14-579-0001-065).

Contribution of the authors to this work: Gordeev AB — bioinformatics analysis of nucleotide sequences, analysis of literature, drafting of a manuscript; Lyubasovskaya LA — micribiological studies, analysis of literature, data interpretation, drafting of a manuscript; Rodchenko JV, Dubodelov DV — microbiological studies, drafting of a manuscript; Mukosey IS, Kochetkova TO — whole genome suquencing and genome assembling; Nikitina IV — research planning, data interpretation; Ionov OB, Zubkov VV — research planning, organization of biomaterial sampling; Trofimov DYu — research planning, organization of whole genome sequencing; Priputnevich TV — research planning, organization of microbiological studies, data interpretation, drafting of a manuscript. All authors participated in editing of the manuscript.

Received: 2017-02-02 Accepted: 2017-02-18 Published online: 2017-03-11
|
  1. Otto M. Molecular basis of Staphylococcus epidermidis infections. Semin Immunopathol. 2012 Mar; 34 (2): 201–14.
  2. Lubasovskaya LA, Kornienko MA, Priputnevich TV, Ilyina EN, Shchegolev AI. [Microbiological and molecular genetic characteristics of coagulase-negative staphylococcal isolates from neonates in intensive care unit]. Antibiot Khimioter. 2013; 58 (3–4): 25–32. Russian.
  3. Miragaia M, Thomas JC, Couto I, Enright MC, de Lencastre H. Inferring a population structure for Staphylococcus epidermidis from multilocus sequence typing data. J Bacteriol. 2007 Mar; 189 (6): 2540–52.
  4. Salgueiro VC, Iorio NL, Ferreira MC, Chamon RC, Dos Santos KR. Methicillin resistance and virulence genes in invasive and nasal Staphylococcus epidermidis isolated from neonates. BMC Microbiol. 2017 Jan 13; 17 (1): 15.
  5. Becker K, Heilmann C, Peters G. Coagulase-negative staphylococci. Clin Microbiol Rev. 2014 Oct; 27 (4): 870–926.
  6. Flamm RK, Mendes RE, Ross JE, Sader HS, Jones RN. An international activity and spectrum analysis of linezolid: ZAAPS Program results for 2011. Diagn Microbiol Infect Dis. 2013 Jun; 76 (2): 206–13.
  7. Mendes RE, Deshpande LM, Costello AJ, Farrell DJ. Molecular epidemiology of Staphylococcus epidermidis clinical isolates from U.S. hospitals. Antimicrob Agents Chemother. 2012 Sep; 56 (9): 4656–61.
  8. McManus BA, Coleman DC, Deasy EC, Brennan GI, O'Connell B, Monecke S, et al. Comparative genotypes, staphylococcal cassette chromosome mec (SCCmec) genes and antimicrobial resistance amongst Staphylococcus epidermidis and Staphylococcus haemolyticus isolates from infections in humans and companion animals. PLoS One. 2015 Sep 17; 10 (9): e0138079.
  9. Dubodelov DV, Lyubasovskaya LA, Schubina ES, Mukosey IS, Korostin DO, Kochetkova TO, et al. Genetic determinants of resistance to beta-lactame antibiotics for Klebsiella pneumoniae clinical strains isolated from newborns. Russ J Genet. 2016; 52: 993–8.
  10. Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H, Marvig RL, et al. Multilocus sequence typing of total-genome- sequenced bacteria. J Clin Microbiol. 2012 Apr; 50 (4): 1355–61.
  11. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012 Nov; 67 (11): 2640–4.
  12. Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, et al. GenBank. Nucleic Acids Res. 2013 Jan; 41 (Database issue): D36–42.
  13. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990 Oct 5; 215 (3): 403–10.
  14. Rohde H, Kalitzky M, Kröger N, Scherpe S, Horstkotte MA, Knobloch JK, et al. Detection of virulence-associated genes not useful for discriminating between invasive and commensal Staphylococcus epidermidis strains from a bone marrow transplant unit. J Clin Microbiol. 2004 Dec; 42 (12): 5614–9.
  15. Thomas JC, Zhang L, Robinson DA. Differing lifestyles of Staphylococcus epidermidis as revealed through Bayesian clustering of multilocus sequence types. Infect Genet Evol. 2014 Mar; 22: 257–64.
  16. Rogers KL, Rupp ME, Fey PD. The presence of icaADBC is detrimental to the colonization of human skin by Staphylococcus epidermidis. Appl Environ Microbiol. 2008 Oct; 74 (19): 6155–7.
  17. Gu J, Li H, Li M, Vuong C, Otto M, Wen Y, et al. Bacterial insertion sequence IS256 as a potential molecular marker to discriminate invasive strains from commensal strains of Staphylococcus epidermidis. J Hosp Infect. 2005 Dec; 61 (4): 342–8.
  18. Conlan S, Mijares LA; NISC Comparative Sequencing Program, Becker J, Blakesley RW, Bouffard GG, et al. Staphylococcus epidermidis pan-genome sequence analysis reveals diversity of skin commensal and hospital infection-associated isolates. Genome Biol. 2012 Jul 25; 13 (7): R64.